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In this study, poly(ethylene glycol) diacrylate (PEGDA), a synthetic polymer at variable concentrations (ranging from 0.2 to 2% wt\/vol) was conjugated with porcine cholecyst derived ECM (C\u2010ECM) (1% wt\/vol) and prepared a biosynthetic hydrogel having enhanced physico\u2010mechanical properties, as required for skeletal muscle tissue engineering. The C\u2010ECM was functionalized with acrylate groups using activated <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide ester\u2010based chemistry and then conjugated with PEGDA <jats:italic>via<\/jats:italic> free\u2010radical polymerization in presence of ammonium persulfate and ascorbic acid. The physicochemical characteristics of the hydrogels were evaluated by Fourier transform infrared spectroscopy and environmental scanning electron microscopy. Further, the hydrogel properties were studied by evaluating rheology, swelling, gelation time, percentage gel fraction, <jats:italic>in vitro<\/jats:italic> degradation, and mechanical strength. Biocompatibility of the gel formulations were assessed using the C2C12 skeletal myoblast cells. The hydrogel formulations containing 0.2 and 0.5% wt\/vol of PEGDA were non\u2010cytotoxic and found suitable for growth and proliferation of skeletal myoblasts. The study demonstrated a method for modulating the properties of ECM hydrogels through conjugation with bio\u2010inert polymers for skeletal muscle tissue engineering applications.<\/jats:p>","DOI":"10.1002\/jbm.a.36955","type":"journal-article","created":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T03:54:50Z","timestamp":1587527690000},"page":"1922-1933","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A cholecystic extracellular matrix\u2010based hybrid hydrogel for skeletal muscle tissue engineering"],"prefix":"10.1002","volume":"108","author":[{"given":"Reshmi","family":"Raj","sequence":"first","affiliation":[{"name":"Division of Experimental Pathology Sree Chitra Tirunal Institute for Medical Sciences and Technology  Thiruvananthapuram 695012 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praveen K.","family":"Sobhan","sequence":"additional","affiliation":[{"name":"Division of 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