{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T13:19:20Z","timestamp":1786367960377,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R15\u2010HL137145"],"award-info":[{"award-number":["R15\u2010HL137145"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1\u2010HL115282"],"award-info":[{"award-number":["RO1\u2010HL115282"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myocardial infarction (MI) results in the death of cardiac tissue, decreases regional contraction, and can lead to heart failure. Tissue engineered cardiac patches containing human induced pluripotent stem cell\u2010derived cardiomyocytes (hiPS\u2010CMs) can restore contractile function. However, cells within thick patches require vasculature for blood flow. Recently, we demonstrated fibronectin coated decellularized leaves provide a suitable scaffold for hiPS\u2010CMs. Yet, the necessity of this additional coating step is unclear. Therefore, we compared hiPS\u2010CM behavior on decellularized leaves coated with collagen IV or fibronectin extracellular matrix (ECM) proteins to noncoated leaves for up to 21\u2009days. Successful coating was verified by immunofluorescence. Similar numbers of hiPS\u2010CMs adhered to coated and noncoated decellularized leaves for 21\u2009days. At Day 14, collagen IV coated leaves contracted more than noncoated leaves (3.25\u2009\u00b1\u20090.39% vs. 1.54\u2009\u00b1\u20090.60%; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). However, no differences in contraction were found between coated leaves, coated tissue culture plastic (TCP), noncoated leaves, or noncoated TCP at other time points. No significant differences were observed in hiPS\u2010CM spreading or sarcomere lengths on leaves with or without coating. This study demonstrates that cardiac scaffolds can be created from decellularized leaves without ECM coatings. Noncoated decellularized leaf surfaces facilitate robust cell attachment for an engineered tissue patch.<\/jats:p>","DOI":"10.1002\/jbm.a.36971","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T02:13:26Z","timestamp":1587608006000},"page":"2123-2132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Creation of a contractile biomaterial from a decellularized spinach leaf without <scp>ECM<\/scp> protein coating: An in vitro study"],"prefix":"10.1002","volume":"108","author":[{"given":"Emily R.","family":"Robbins","sequence":"first","affiliation":[{"name":"Biomedical Engineering Worcester Polytechnic Institute  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George D.","family":"Pins","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Worcester Polytechnic Institute  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Laflamme","sequence":"additional","affiliation":[{"name":"McEwen Stem Cell Institute University Health Network  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn R.","family":"Gaudette","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Worcester Polytechnic Institute  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1980.239.3.H432"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2004.12.011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000485"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14128"},{"key":"e_1_2_7_6_1","first-page":"1","article-title":"Vascular perfusion of implanted human engineered cardiac tissue","volume":"2014","author":"Coulombe K. 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