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Wear particles are well known to be the initiative cause inducing osteolysis through enhancing osteoclast\u2010mediated bone resorption and reducing osteogenic differentiation. The purpose of this study was to investigate the effects of osteoclast\u2010secreted exosomal long noncoding RNAs (lncRNAs) on osteogenesis in the process of particle\u2010induced osteolysis. RAW264.7 cells were treated by titanium particles (TI). The inflammatory cytokines were increased, and expression of Receptor Activator of Nuclear Factor\u2010\u03baB and Nuclear factor of activated T cells c1 were also increased, indicating osteoclast differentiation occurred. The purified exosomes from RAW264.7 cells induced with TI inhibited osteogenic differentiation of MC3T3\u2010E1 cells. RNA sequencing generated lncRNAs expression profiles (458 up\u2010regulated and 1641 down\u2010regulated) of the exosomes derived from RAW264.7 cells treated with TI. Based on the results of gene ontology\/Kyoto Encyclopedia of Genes and Genomes analysis and quantitative real time polymerase chain reaction validation, we confirmed two candidate lncRNAs, NONMMUT000375.2 and NONMMUT071578.2. The regulation network presented that some vital genes involved in osteoclast differentiation, such as Bcl2, Wnt11, TGF\u2010\u03b2, and Pdk1, were under the regulation of NONMMUT000375.2 and NONMMUT071578.2. Taken together, exosomes derived from TI treated RAW264.7 cells inhibit the osteogenic activity of MC3T3\u2010E1 cells. Exosomal lncRNAs, NONMMUT000375.2 and NONMMUT071578.2 may potentially play their roles in promoting osteoclast differentiation and suppressing osteogenesis, which aggravates the osteoclastogenesis\/osteogenesis imbalance.<\/jats:p>","DOI":"10.1002\/jbm.a.36983","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T02:03:30Z","timestamp":1588557810000},"page":"2251-2262","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Exosomal\n                    <scp>lncRNAs NONMMUT000375<\/scp>\n                    .2 and\n                    <scp>NONMMUT071578<\/scp>\n                    .2 derived from titanium particle treated\n                    <scp>RAW264<\/scp>\n                    .7 cells regulate osteogenic differentiation of\n                    <scp>MC3T3\u2010E1<\/scp>\n                    cells"],"prefix":"10.1002","volume":"108","author":[{"given":"Jie","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Joint Surgery Sun Yat\u2010sen Memorial Hospital, Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery Sun Yat\u2010sen Memorial Hospital, Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqing","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery Sun Yat\u2010sen Memorial Hospital, Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery Sun Yat\u2010sen Memorial Hospital, Sun Yat\u2010sen University  Guangzhou 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