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Nanoparticle therapeutics have the potential to improve treatment of these infections. Here we report the development of liposomal nanoparticles incorporating anidulafungin, a potent antifungal, with the goal of increasing its solubility and aiding in localization to fungi. Liposomes were fabricated with three concentrations of anidulafungin yielding monodisperse ~100\u2009nm unilamellar vesicles. All three formulations inhibited planktonic <jats:italic>Candida albicans<\/jats:italic> growth at a minimum inhibitory concentration equivalent to free drug. All three formulations also disrupted preformed <jats:italic>C. albicans<\/jats:italic> biofilms, reducing fungal burden by as much as 99%, exhibiting superior biofilm disruption compared with free drug. Liposome formulations tested in vivo in <jats:italic>C. albicans<\/jats:italic> infected <jats:italic>Galleria mellonella<\/jats:italic> wax moth larvae demonstrated increased survival compared to free drug equivalents, leading to a survival of 33 to 67% of larvae over 7\u2009days depending on the liposome utilized compared with only 25% survival of larvae administered free drug. Liposomal formulations along with free anidulafungin did not cause red blood cell lysis. Ultimately, the liposome formulations reported here increased anidulafungin solubility, displayed promising efficacy against planktonic and biofilm <jats:italic>C. albicans<\/jats:italic>, and improved the survival of <jats:italic>C. albicans<\/jats:italic>\u2013infected <jats:italic>G. mellonella<\/jats:italic> compared to free anidulafungin.<\/jats:p>","DOI":"10.1002\/jbm.a.36984","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T06:19:21Z","timestamp":1588573161000},"page":"2263-2276","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm <scp><i>Candida albicans<\/i><\/scp>"],"prefix":"10.1002","volume":"108","author":[{"given":"Noel","family":"Vera\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"School of Engineering, Center for Biomedical Engineering, Institute for 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