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The encapsulation efficiency was found to be 69.54%\u2009\u00b1;\u20090.67. The prepared niosomal formulations had a high storage stability to 60\u2009days with small changes in size and drug entrapment, which indicates that it is a suitable candidate for pharmaceutical applications. The results of biological study showed the anti\u2010bacterial activity via reduction of antibiotic resistance, enhanced anti\u2010efflux and anti\u2010biofilm activities by more folds in comparison to free tobramycin. In addition, niosome encapsulated tobramycin down\u2010regulated the <jats:italic>MexAB\u2010OprM<\/jats:italic> efflux genes, <jats:italic>pslA<\/jats:italic> and <jats:italic>pelA<\/jats:italic> biofilm related genes in MDR <jats:italic>P. aeruginosa<\/jats:italic> strains. The anti\u2010proliferative activity of formulation was evaluated against HEK293 cell lines, which exhibited negligible cytotoxicity against HEK293 cells. The finding of our study shows that encapsulation of tobramycin in niosome enhanced the antibacterial activity and reduced antibiotic resistance in MDR strains of <jats:italic>P. aeruginosa<\/jats:italic> comparing to free tobramycin and it can be considered as a favorable drug delivery system.<\/jats:p>","DOI":"10.1002\/jbm.a.37086","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T13:34:18Z","timestamp":1598880858000},"page":"966-980","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Niosome\u2010encapsulated tobramycin reduced antibiotic resistance and enhanced antibacterial activity against multidrug\u2010resistant clinical strains of <scp><i>Pseudomonas aeruginosa<\/i><\/scp>"],"prefix":"10.1002","volume":"109","author":[{"given":"Mojtaba","family":"Hedayati Ch","sequence":"first","affiliation":[{"name":"Department of Microbiology Guilan University of Medical Sciences  Rasht 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