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Tissue engineered islets are promising alternatives to traditional organ transplantation. Recent progress in stem cell biology and gene cloning techniques has raised hopes for the generation of insulin producing cells (IPCs) without the need of immunosuppression. The purpose of this study was to produce IPCs using human adipose\u2010derived stem cells (hADSCs) and human endometrial\u2010derived stem cells (hEnSCs) and also to compare the level of insulin secretion by these cells in 2D and 3D culture systems on fibrin scaffolding. Stem cells differentiation was carried out through transduction with an insulin over expression lentiviral vector. Real\u2010time PCR and immunocytochemistry confirmed the successful transduction of both cell types. Both cell types showed comparable insulin secretion by ELISA.3D culture resulted in higher amounts of insulin secretion of the two cell types versus 2D as control. This study showed that insulin gene delivery to the stem cells could be an efficient method for producing IPCs and fibrin encapsulation enhances the functionality of these cells.<\/jats:p>","DOI":"10.1002\/jbm.a.37094","type":"journal-article","created":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T09:04:26Z","timestamp":1598778266000},"page":"1036-1044","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of insulin secretion by transduced adipose\u2010derived and endometrial\u2010derived stem cells in <scp>2D<\/scp> and <scp>3D<\/scp> cultures on fibrin scaffold"],"prefix":"10.1002","volume":"109","author":[{"given":"Roya","family":"Karimi","sequence":"first","affiliation":[{"name":"Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine Tehran University of Medical Sciences  Tehran 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