{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:22:33Z","timestamp":1790770953974,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,3,7]],"date-time":"2021-03-07T00:00:00Z","timestamp":1615075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2018M643504"],"award-info":[{"award-number":["2018M643504"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Whole\u2010organ engineering is emerging as an alternative source for xenotransplantation in end\u2010stage diseases. Utilization of decellularized whole lung scaffolds created by detergent perfusion is an effective strategy for organ replacement. In the current study, we attempted to decellularize porcine whole lungs to generate an optimal and reproducible decellularized matrix for future clinical use. Porcine whole lungs were decellularized via perfusion of various detergents (sodium dodecyl sulfate (SDS)\/Triton X\u2010100, sodium lauryl ether sulfate (SLES)\/Triton X\u2010100, dextrose\/SDS\/Triton X\u2010100 and dextrose\/SLES\/Triton X\u2010100) through the pulmonary artery and bronchus of the lung. The decellularized scaffolds were evaluated for decellularization efficiency, extracellular matrix (ECM) component preservation, xenoantigen removal and compatibility. The resulting lung scaffolds obtained from treatment with the dextrose\/SLES\/Triton X\u2010100 cocktail showed minimal residual cellular components and xenoantigens, including DNA and protein, and good preservation of ECM composition. Evaluation of the porcine lung ECM by specific staining and immunofluorescence confirmed that the three\u2010dimensional ultrastructure of the ECM was noticeably preserved in the SLES\u2010treated groups. In addition, the decellularized lung scaffolds originating from the dextrose\/SLES\/Triton X\u2010100 cocktail supported cell adhesion and growth. In summary, the novel detergent SLES alleviated the damage to retain a better\u2010preserved ECM than SDS. Sequential Triton X\u2010100 perfusion eliminated SLES. Moreover, performing dextrose perfusion in advance further protected scaffold components, especially collagen. We developed an optimal dextrose\/SLES\/Triton X\u2010100 cocktail method that can be used for the decellularization of porcine whole lung to obtain a clinical\u2010scale bioengineered scaffold.<\/jats:p>","DOI":"10.1002\/jbm.a.37158","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T01:05:02Z","timestamp":1615165502000},"page":"1623-1632","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Decellularization of porcine whole lung to obtain a clinical\u2010scale bioengineered scaffold"],"prefix":"10.1002","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8661-4617","authenticated-orcid":false,"given":"Yi","family":"Li","sequence":"first","affiliation":[{"name":"Precision Medicine Key Laboratory, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8413-3270","authenticated-orcid":false,"given":"Ji","family":"Bao","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0985-0311","authenticated-orcid":false,"given":"Weimin","family":"Li","sequence":"additional","affiliation":[{"name":"Precision Medicine Key Laboratory, West China Hospital Sichuan University  Chengdu Sichuan China"},{"name":"Department of Respiratory and Critical Care Medicine, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.14-1748"},{"key":"e_1_2_8_3_1","unstructured":"Mao W.andJ.Chen Difficulties and countermeasures of lung transplantation in China. 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