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However, thrombosis poses a great challenge that hinders the transplantation for a substantial organ in vivo. Therefore, anticoagulation and re\u2010reendothelialization of organ biological scaffolds are the primary concerns to be addressed before orthotopic transplantation. Herein, a heparinized decellularized kidney scaffold (HEP\u2010DKSs) was prepared using end\u2010point attachment technology, followed by binding the vascular endothelial growth factor (VEGF) to greatly improve the hemocompatibility and angiogenesis of DKSs. Based on the anticoagulant, co\u2010culture of human umbilical vein endothelial cells, and subcapsular transplantation of kidney experiments, HEP\u2010VEGF\u2010DKSs are shown to reduce platelet adhesion, which is crucial for subsequent vascularization and slow release of heparin and VEGF, suggesting its ability of improve neovascularization. Taken together, these data indicated an optimal anticoagulation function of HEP\u2010VEGF\u2010DKSs and the potential of vascularization for regeneration of whole decellularized kidney.<\/jats:p>","DOI":"10.1002\/jbm.a.37190","type":"journal-article","created":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T10:47:50Z","timestamp":1617706070000},"page":"1979-1989","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effect of heparinization on promoting angiogenesis of decellularized kidney scaffolds"],"prefix":"10.1002","volume":"109","author":[{"given":"Lebin","family":"Zhou","sequence":"first","affiliation":[{"name":"Anatomy Department Wenzhou Medical University  Wenzhou China"},{"name":"Department of Emergency People's Hospital of Yueqing  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General 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