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To better predict the outcome of FGF\u20102 supplementation, we investigated whether endogenous levels of FGF\u20102 play a role in optimal dosing of FGF\u20102 for augmenting BMP\u20102 activity in elderly mice. In vivo calvarial bone defect studies in <jats:italic>Fgf2<\/jats:italic> knockout mice with wildtype controls were conducted with the growth factors delivered in a highly localized manner from a biomimetic calcium phosphate\/polyelectrolyte multilayer coating applied to a bone graft substitute. Endogenous FGF\u20102 levels were measured in old mice versus young and found to decrease with age. Optimal dosing for improving bone defect repair correlated with levels of endogenous FGF\u20102, with a larger dose of FGF\u20102 required to have a positive effect on bone healing in the <jats:italic>Fgf2<\/jats:italic> knockout mice. The same dose in wildtype old mice, with higher levels of FGF\u20102, promoted chondrogenesis and increased osteoclast activity. The results suggest a personalized medicine approach, based on a knowledge of endogenous levels of FGF\u20102, should guide FGF\u20102 supplementation in order to avoid provoking excessive bone resorption and cartilage formation, both of which inhibited calvarial bone repair.<\/jats:p>","DOI":"10.1002\/jbm.a.37249","type":"journal-article","created":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T10:30:59Z","timestamp":1624703459000},"page":"2545-2555","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Endogenous <scp>FGF<\/scp>\u20102 levels impact <scp>FGF<\/scp>\u20102\/<scp>BMP<\/scp>\u20102 growth factor delivery dosing in aged murine calvarial bone defects"],"prefix":"10.1002","volume":"109","author":[{"given":"Liisa T.","family":"Kuhn","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Connecticut Health Center  Farmington Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Connecticut Health Center  Farmington Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Gronowicz","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Connecticut Health Center  Farmington Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja M","family":"Hurley","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Connecticut Health Center  Farmington Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2008.00377.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(03)00155-4"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.050503"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24792"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2019.110643"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2015.02.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35211"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.11607\/prd.2633"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.12.009"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001665-199611000-00006"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908531107"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.03.067"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.074"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.10.052"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35985"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glt018"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.5435\/JAAOS-22-10-677"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(02)00046-8"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.12.031"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-019-00993-2"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.61.869"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2015.0357"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21217967"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2017.0111"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.04.013"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(97)00292-5"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(94)00049-2"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2016.21"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0537"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.1405"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.052"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0298-201"},{"key":"e_1_2_7_34_1","unstructured":"National Institutes of Aging.https:\/\/www.nia.nih.gov\/research\/dab\/aged-rodent-colonies-handbook\/strain-survival-information. 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