{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T16:48:56Z","timestamp":1775926136492,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T00:00:00Z","timestamp":1639612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to select the optimal delipidation solvent for preparation of human perirenal adipose tissue\u2010derived extracellular matrix (ECM). Human perirenal adipose tissue can be obtained in large amounts during surgery, and it can be an alternative source of human ECM. Delipidation is an essential procedure for the ECM preparation, because lipid strongly inhibits regeneration of target tissue. Isopropanol has been widely used as a delipidation solvent for adipose tissue. However, because adipose tissue is mostly composed of nonpolar lipid, a nonpolar solvent might be more effective for delipidation. We evaluated the delipidation efficiency of acetone, chloroform, methanol, ether, ethanol, isopropanol, water, chloroform\/methanol, ethanol\/heptane, ether\/methanol, hexane\/ethanol, and butanol\/methanol solvents for ECM extraction from human perirenal adipose tissue. Among them, acetone\u2010treated adipose tissue showed the greatest delipidation efficiency (93.05%), significantly lower residual DNA content, and the greatest residual collagen concentration (42.49\u2009\u00b1\u20090.05\u2009\u03bcg\/g). In addition, acetone\u2010treated tissue also had well\u2010preserved ultrastructure with high porosity and significantly low in vitro cytotoxicity. These results suggested that acetone may be an optimal delipidation solvent for extraction of ECM from human perirenal adipose tissue.<\/jats:p>","DOI":"10.1002\/jbm.a.37341","type":"journal-article","created":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T14:16:30Z","timestamp":1639664190000},"page":"928-942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Optimal delipidation solvent to secure extracellular matrix from human perirenal adipose tissue"],"prefix":"10.1002","volume":"110","author":[{"given":"So Young","family":"Chun","sequence":"first","affiliation":[{"name":"BioMedical Research Institute Kyungpook National University Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Sok","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine Kyungpook National University, Kyungpook National University Chilgok Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6838-9313","authenticated-orcid":false,"given":"Bo Hyun","family":"Yoon","sequence":"additional","affiliation":[{"name":"Joint Institute of Regenerative Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Hye","family":"Lee","sequence":"additional","affiliation":[{"name":"Joint Institute of Regenerative Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"BioMedical Research Institute Kyungpook National University Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haejung","family":"Gil","sequence":"additional","affiliation":[{"name":"BioMedical Research Institute Kyungpook National University Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man\u2010Hoon","family":"Han","sequence":"additional","affiliation":[{"name":"Joint Institute of Regenerative Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Gyun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine Kyungpook National University, Kyungpook National University Chilgok Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bum Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Kyungpook National University, Kyungpook National University Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun Nyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine Kyungpook National University, Kyungpook National University Chilgok Hospital  Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2009.0276"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.8672"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2334-1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00193-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2009.00796.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-018-6164-z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(94)90036-1"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(93)90238-W"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(95)70039-0"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.33056"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-002-1004-1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(94)90162-7"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00217-7"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0843-0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-013-9391-z"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.014"},{"key":"e_1_2_9_18_1","unstructured":"FlynnLE.Decellularized adipose tissue. 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