{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T09:33:23Z","timestamp":1781602403444,"version":"3.54.5"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["F31 DE030333"],"award-info":[{"award-number":["F31 DE030333"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P41 EB023833"],"award-info":[{"award-number":["P41 EB023833"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AR068073"],"award-info":[{"award-number":["R01 AR068073"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thermogelling hydrogels based on poly(<jats:italic>N<\/jats:italic>\u2010isopropyl acrylamide) (p[NiPAAm]) and crosslinked with a peptide\u2010bearing macromer poly(glycolic acid)\u2010poly(ethylene glycol)\u2010poly(glycolic acid)\u2010di(but\u20102\u2010yne\u20101,4\u2010dithiol) (PdBT) were fabricated to assess the role of hydrogel charge and lower critical solution temperature (LCST) over time in influencing cellular infiltration and tissue integration in an ex vivo cartilage explant model over 21\u2009days. The p(NiPAAm)\u2010based thermogelling polymer was synthesized to possess 0, 5, and 10\u00a0mol% dimethyl\u2010\u03b3\u2010butyrolactone acrylate (DBA) to raise the LCST over time as the lactone rings hydrolyzed. Further, three peptides were designed to impart charge into the hydrogels via conjugation to the PdBT crosslinker. The positively, neutrally, and negatively charged peptides K4 (+), zwitterionic K2E2 (0), and E4 (\u2212), respectively, were conjugated to the modular PdBT crosslinker and the hydrogels were evaluated for their thermogelation behavior in vitro before injection into the cartilage explant models. Samples were collected at days 0 and 21, and tissue integration and cellular infiltration were assessed via mechanical pushout testing and histology. Negatively charged hydrogels whose LCST changed over time (10\u00a0mol% DBA) were demonstrated to promote the greatest tissue integration when compared to the positive and neutral gels of the same thermogelling polymer formulation due to increased transport and diffusion across the hydrogel\u2010tissue interface. Indeed, the negatively charged thermogelling polymer groups containing 5 and 10\u00a0mol% DBA demonstrated cellular infiltration and cartilage\u2010like matrix deposition via histology. This study demonstrates the important role that material physicochemical properties play in dictating cell and tissue behavior and can inform future cartilage tissue engineering strategies.<\/jats:p>","DOI":"10.1002\/jbm.a.37441","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T04:02:16Z","timestamp":1661832136000},"page":"15-34","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Thermogelling hydrogel charge and lower critical solution temperature influence cellular infiltration and tissue integration in an ex vivo cartilage explant model"],"prefix":"10.1002","volume":"111","author":[{"given":"Hannah A.","family":"Pearce","sequence":"first","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph W. R.","family":"Swain","sequence":"additional","affiliation":[{"name":"Depatment of Chemistry Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Hector","family":"Victor","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie J.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily Y.","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew L.","family":"Bedell","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam M.","family":"Navara","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Farsheed","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"},{"name":"Depatment of Chemistry Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Seon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason L.","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D.","family":"Hartgerink","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"},{"name":"Depatment of Chemistry Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Jane","family":"Grande\u2010Allen","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonios G.","family":"Mikos","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Rice University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.09.048"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.07.041"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.18-0065"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.03.030"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.04.038"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.07.006"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.06.035"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36848"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27804"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm401413c"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm300797m"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw7396"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm300429e"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.12.026"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.09.084"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.01.012"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0TB00172D"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbab012"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbab073"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.6b00990"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00176-X"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201701199"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2020.123063"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.02.115"},{"key":"e_1_2_8_26_1","first-page":"1","article-title":"Condensation\u2010driven chondrogenesis of human mesenchymal stem cells within their own extracellular matrix: formation of cartilage with low hypertrophy and physiologically relevant mechanical properties","volume":"3","author":"Yang Y","year":"2019","journal-title":"Adv Biosyst"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11538-013-9897-3"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748\u20106041\/7\/2\/024110"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856209X451323"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.9b01450"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.8b01348"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800040"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/prep.19920170503"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.28.2254"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.12.027"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2473"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm4009606"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2022.105391"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e3181d2526d"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.09.28"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmacrolett.0c00520"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202005946"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1039\/b919944f"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ab3582"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.06.001"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v027sa03"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-016-1130-8"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma9100854"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31098"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp020287z"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2018.1447627"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1114\/1.1581879"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1089\/107632703762687492"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.12.008"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.11.028"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(85)90401-4"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-200300002-00019"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(200002)49:2<200::AID-JBM7>3.0.CO;2-L"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.03.013"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10098"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.07.022"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.08.073"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.37441","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.a.37441","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/jbm.a.37441","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.37441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T17:12:26Z","timestamp":1692465146000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.37441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,29]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/jbm.a.37441"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.37441","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,29]]},"assertion":[{"value":"2022-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}