{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:39:32Z","timestamp":1790753972129,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T00:00:00Z","timestamp":1665360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005440","name":"Center for Scientific Review","doi-asserted-by":"publisher","award":["NIH 1R15AR071102"],"award-info":[{"award-number":["NIH 1R15AR071102"]}],"id":[{"id":"10.13039\/100005440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biomimetic scaffolds composed of bioactive ceramic\u2010based materials incorporated within a polymeric framework have shown immense promise for use in bone tissue engineering (BTE) applications. However, studies on direct comparison of the efficacy of different bioceramics on bone bioactivity and osteogenic differentiation are lacking. Herein, we performed an <jats:italic>in vitro<\/jats:italic> direct comparison of three different bioceramics\u2014Bioglass 45S5 (BG), Laponite XLG (LAP), and \u03b2\u2010Tricalcium Phosphate (TCP)\u2014on the physical properties and bone bioactivity of methacrylated collagen (CMA) hydrogels (10% w\/w bioceramic:CMA). In addition, human MSCs (hMSCs) were encapsulated in bioceramic\u2010laden CMA hydrogels and the effect of different bioceramics on osteogenic differentiation of hMSCs was investigated in two different culture medium\u2014osteoconductive (without dexamethasone [DEX]) and osteoinductive (with DEX). Results showed that the stability of CMA hydrogels was maintained upon bioceramic addition. Compression testing revealed that BG incorporation significantly decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the modulus of photochemically crosslinked CMA hydrogels. Incubation of TCP\u2010CMA and LAP\u2010CMA hydrogels in simulated body fluid showed deposition of hydroxycarbonate apatite layer on the surface indicating that these hydrogels may be more bone bioactive than BG\u2010CMA and CMA only hydrogels. Cell cytoskeleton staining results showed greater cell spreading in TCP\u2010CMA hydrogels. Furthermore, TCP incorporation significantly increased alkaline phosphatase activity (ALP; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in hMSCs. Together, these results indicate that TCP has superior osteogenic potential compared with BG and LAP and hence should be considered as a bioceramic of preferred choice for use in the biomimetic design of cell\u2010laden hydrogels for BTE applications.<\/jats:p>","DOI":"10.1002\/jbm.a.37452","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T14:44:10Z","timestamp":1665413050000},"page":"224-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A comparative study of bone bioactivity and osteogenic potential of different bioceramics in methacrylated collagen hydrogels"],"prefix":"10.1002","volume":"111","author":[{"given":"Nashaita Y.","family":"Patrawalla","sequence":"first","affiliation":[{"name":"Department of Biomedical and Chemical Engineering and Sciences Florida Institute of Technology  Melbourne Florida 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