{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T11:06:49Z","timestamp":1789038409217,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T00:00:00Z","timestamp":1668902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007780","name":"Indian Institute of Science","doi-asserted-by":"publisher","award":["IE\/RERE\u201021\u20100528"],"award-info":[{"award-number":["IE\/RERE\u201021\u20100528"]}],"id":[{"id":"10.13039\/100007780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study reports the impact of the interplay between electroactive properties of the biomaterials and electrical stimulation (ES) toward the cell proliferation, migration and maturation of osteoprogenitors (preosteoblasts; MC3T3\u2010E1) on the electroactive poly (vinylidene difluoride) (PVDF)\u2010based composites. The barium titanate (BaTiO<jats:sub>3;<\/jats:sub> BT; 30\u2009wt%) and multiwalled carbon nanotubes (MWCNT; 3\u00a0wt%) were introduced into the PVDF via melt mixing, which led to an enhancement of the dielectric permittivity, electrical conductivity, and surface roughness. We also present the design and development of an in\u2010house customized 12\u2010well plate\u2010based device for providing different types (DC, square, biphasic) of ES to cells in culture in a programmable manner. In the presence of ES of 1\u00a0V\u2009cm<jats:sup>\u22121<\/jats:sup>, biophysical stimulation experiments performed using 12\u2010well plate\u2010based device revealed that PVDF composite (PVDF\/30BT\/3MWCNT) can facilitate the enhanced adhesion and proliferation of the MC3T3\u2010E1 in non\u2010osteogenic media, with respect to non\u2010stimulated conditions. Importantly, MC3T3\u2010E1 cells demonstrated significantly better migration and differentiation on the PVDF\/30BT\/3MWCNT under ES when compared to ES\u2010free culture conditions. Similar enhancement with respect to alkaline phosphatase activity, intracellular Ca<jats:sup>2+<\/jats:sup> concentration, and calcium deposition in MC3T3\u2010E1 cells was recorded, when pre\u2010osteoblasts were grown for 21\u2009days on electroactive substrates. All these observations supported the activation of osteo\u2010differentiation fates, which were further promoted in the osteogenic medium. The present study demonstrates that the synergistic interactions of ES with piezoelectric PVDF\u2010based polymer composite can potentially enhance the proliferation, migration, and osteogenesis of the pre\u2010osteoblast cells, rendering it a promising bioengineering strategy for bone tissue engineering.<\/jats:p>","DOI":"10.1002\/jbm.a.37472","type":"journal-article","created":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T19:46:46Z","timestamp":1668973606000},"page":"340-353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Electrical field stimulated modulation of cell fate of pre\u2010osteoblasts on <scp>PVDF<\/scp>\/<scp>BT<\/scp>\/<scp>MWCNT<\/scp> based electroactive biomaterials"],"prefix":"10.1002","volume":"111","author":[{"given":"Nitu","family":"Bhaskar","sequence":"first","affiliation":[{"name":"Laboratory for Biomaterials Materials Research Centre, Indian Institute of Science  Bangalore 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