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Acrylate\u2010derivatized PEG hydrogels (PEGDA) are some of the most widely studied hydrogels; however, there has been debate around the degradation mechanism and predicting resorption rates. Several factors influence the degradation rate of PEG hydrogels, including backbone and endgroup chemistry, macromer molecular weight, and polymer concentration. In addition to hydrogel parameters, it is necessary to understand the influence of biological and environmental conditions (e.g., pH and temperature) on hydrogel degradation. Rigorous methods for monitoring degradation in both in vitro and in vivo settings are also critical to hydrogel design and development. Herein, we provide guidance on tailoring PEG hydrogel chemistry to achieve target hydrolytic degradation kinetics for both resorbable and biostable applications. A detailed overview of accelerated testing methods and hydrogel degradation characterization is provided to aid researchers in experimental design and interpreting in vitro\u2013in vivo correlations necessary for predicting hydrogel device performance.<\/jats:p>","DOI":"10.1002\/jbm.a.37609","type":"journal-article","created":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T06:16:23Z","timestamp":1694844983000},"page":"1200-1212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["A user's guide to degradation testing of polyethylene glycol\u2010based hydrogels: From <i>in vitro<\/i> to <i>in vivo<\/i> studies"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6022-2279","authenticated-orcid":false,"given":"Gabriel J.","family":"Rodriguez\u2010Rivera","sequence":"first","affiliation":[{"name":"McKetta Department of Chemical Engineering The University of Texas  Austin Texas 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