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This preliminary study demonstrates that surface immobilized \u03b1\u20101 acid glycoprotein (AGP), as well as collagen VI (Col6) in conjunction with AGP, can direct macrophages towards the M2 polarization state in vitro and modify the foreign body response in vivo<jats:italic>.<\/jats:italic> AGP and Col6 are immobilized onto poly(2\u2010hydroxyethyl methacrylate) (pHEMA) surfaces using carbonyl diimidazole chemistry. Mouse bone marrow derived macrophages are cultured on modified surfaces with or without lipopolysaccharide stimulation. Surface modified pHEMA discs are implanted subcutaneously into mice to observe differences in the foreign body response. After stimulation with lipopolysaccharide, macrophages cultured on AGP or Col6 modified surfaces showed a reduction in TNF\u2010\u03b1 expression compared to controls. Arg1 expression was also increased in macrophages cultured on modified surfaces. Explanted tissues showed that the foreign body capsule around implants with AGP or AGP and Col6 modification had reduced thickness, while also being more highly vascularized. These data demonstrate that \u03b1\u20101 acid glycoprotein and collagen VI could potentially be used for the surface modification of medical devices to influence macrophage polarization leading to a reduced and modulated foreign body response.<\/jats:p>","DOI":"10.1002\/jbm.a.37627","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T11:19:16Z","timestamp":1698232756000},"page":"1241-1249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Surface immobilized \u03b1\u20101 acid glycoprotein and collagen <scp>VI<\/scp> modulate mouse macrophage polarization and reduce the foreign body capsule"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-2723","authenticated-orcid":false,"given":"Alex C.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Bioengineering University of Washington  Seattle Washington 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