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Thus, there is a growing interest in developing advanced monoclonal antibody delivery systems that can customize pharmacokinetics to enhance therapeutic outcomes. This work aimed to engineer hydrolytic 4\u2010arm PEG maleimide (PEG\u20104MAL) microgels for the controlled delivery of therapeutic antibodies, specifically anti\u2010angiogenic bevacizumab, to overcome the limitations of current monoclonal antibody therapies. Through a PEGylation reaction with a thiol\u2010terminated PEG linker, the antibody was covalently conjugated to the macromer backbone before microgel synthesis. The PEGylation reaction was simple, effective, and did not affect antibody bioactivity. Antibody release kinetics was tuned by changing the concentration of the hydrolytic linker (0\u20132\u2009mM) and\/or PEG\u20104MAL:protein molar ratio (1000:1, 2000:1, and 5000:1) in the macromer precursor solution during microgel fabrication. The bioactivity of the released antibody was assessed on human umbilical endothelial vascular cells (HUVEC), demonstrating that extracts from hydrolytic microgels reduced cell proliferation over time. Collectively, this study demonstrates the development of highly tunable delivery platform based on degradable PEG\u20104MAL microgels that can be adapted for therapeutic antibody\u2010controlled release.<\/jats:p>","DOI":"10.1002\/jbm.a.37637","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T07:26:39Z","timestamp":1699255599000},"page":"1265-1275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Controlled release of therapeutic antibody using hydrolytically degradable microgels"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4366-6860","authenticated-orcid":false,"given":"Ana","family":"Mora\u2010Boza","sequence":"first","affiliation":[{"name":"Petit Institute for Bioengineering and Biosciences Georgia Institute of Technology  Atlanta Georgia USA"},{"name":"Woodruff School of Mechanical Engineering Georgia Institute of Technology  Atlanta 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