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Sterilization is a key step before the application of graft, and irradiation is one of the most commonly used methods. In this paper, APC was prepared by the physical freeze\u2010thawing combined with biological enzymes, and the effects of the electron beam (E\u2010beam) and cobalt 60 (<jats:sup>60<\/jats:sup>Co\u2010\u03b3) at the dose of 15\u2009kGy on the physicochemical properties, structure, immunogenicity, and biocompatibility of the APC were investigated. After decellularization, the residual DNA was 20.86\u2009\u00b1\u20091.02\u2009ng\/mg, and the \u03b1\u2010Gal clearance rate was more than 99%. Irradiation, especially the <jats:sup>60<\/jats:sup>Co\u2010\u03b3, reduced the cornea's transmittance, elastic modulus, enzymatic hydrolysis rate, swelling ratio, and cross\u2010linking degree. Meanwhile, the diameter and spacing of the collagen fibers increased. In the rat subcutaneous implantation, many inflammatory cells appeared in the unirradiated APC, while the irradiated had good histocompatibility, but the degradation was faster. The lamellar keratoplasty in rabbits indicated that compared to the E\u2010beam, the <jats:sup>60<\/jats:sup>Co\u2010\u03b3 damaged the chemical bond of collagen to a larger extent, reduced the content of GAGs, and prolonged the complete epithelization of the grafts. The corneal edema was more serious within 1\u2009month after the surgery. After 2\u2009months, the thickness of the APC with the two irradiation methods tended to be stable, but that in the <jats:sup>60<\/jats:sup>Co\u2010\u03b3 group became thinner. The pathological results showed that the collagen structure was looser and the pores were larger, indicating the <jats:sup>60<\/jats:sup>Co\u2010\u03b3 had a more extensive effect on the APC than the E\u2010beam at 15\u2009kGy.<\/jats:p>","DOI":"10.1002\/jbm.a.37663","type":"journal-article","created":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T12:13:40Z","timestamp":1703938420000},"page":"825-840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structure and properties of the acellular porcine cornea irradiated with <sup>60<\/sup>Co\u2010\u03b3 and electron beam and its histocompatibility"],"prefix":"10.1002","volume":"112","author":[{"given":"Qing","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory for Liquid\u2010Solid Structural Evolution and Processing of Materials (Ministry of Education), School of Materials Science and Engineering Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Sun","sequence":"additional","affiliation":[{"name":"Qingdao Chunghao Tissue Engineering Co., Ltd.  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