{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:37:36Z","timestamp":1789565856538,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011821","name":"Ministry of Education \u2013 Kingdom of Saudi Arabi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011821","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Stimuli\u2010responsive domains capable of releasing loaded molecules, \u201con\u2010demand,\u201d have garnered increasing attention due to their enhanced delivery, precision targeting, and decreased adverse effects. The development of an on\u2010demand delivery system that can be easily triggered by dental clinicians might have major roles in dental and oral tissue engineering. A series of random graft poly(NIPAm\u2010co\u2010HEMA\u2010Lactate) copolymers were synthesized using 95:5, 85:5, 60:40, and 40:60 ratios of thermosensitive NIPAm and HEMA\u2010poly lactate respectively then electrospun to produce nanofibrous scaffolds loaded with bovine serum albumin (BSA). Cumulative BSA release was assessed at 25C and 37\u00b0C. To appraise the use of scaffolds as on\u2010demand delivery systems, they were subjected to thermal changes in the form cooling and warming cycles during which BSA release was monitored. To confirm the triggered releasing ability of the synthesized scaffolds, the copolymer made with 60% NIPAm was selected, based on the results of the release tests, and loaded with bone morphogenetic protein\u20102 (BMP\u20102). The loaded scaffolds were placed with mesenchymal\u2010like stem cells (iMSCs) derived from induced pluripotent stem cells (iPSCs), and subjected to temperature alterations. Then, the osteogenic differentiation of iMSCs, which might have resulted from the released protein, was evaluated after 10\u2009days by analyzing runt\u2010related transcription factor 2 (RUNX\u20102) osteogenic gene expression by the cells using real\u2010time quantitative polymerase chain reaction (qRT\u2010PCR). BSA release profiles showed a burst release at the beginning followed by a more linear pattern at 25\u00b0C, and a much slower release at 37\u00b0C. The release also decreased when the PNIPAm content decreased in the scaffolds. Thermal triggering led to a step\u2010like release pattern in which the highest release was reported 30\u2009min through the warming cycles. The iMSCs cultivated with scaffolds loaded with BMP\u20102 and exposed to temperature alteration showed significantly higher RUNX\u20102 gene expression than cells in the other experimental groups. The synthesized scaffolds are thermo\u2010responsive and could be triggered to deliver biological biomolecules to be used in oral and dental tissue engineering. Thermal stimuli could be simulated by dental clinicians using simple means of cold therapy, for example, cold packs in intraoral accessible sites for specified times.<\/jats:p>","DOI":"10.1002\/jbm.a.37664","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T02:10:31Z","timestamp":1704766231000},"page":"852-865","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Poly(N\u2010isopropylacrylamide) based smart nanofibrous scaffolds for use as on\u2010demand delivery systems for oral and dental tissue regeneration"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8320-7186","authenticated-orcid":false,"given":"Dina S.","family":"Farahat","sequence":"first","affiliation":[{"name":"Department of Biologic and Materials Sciences and Prosthodontics, School of Dentistry University of Michigan  Ann Arbor Michigan USA"},{"name":"Department of Dental Biomaterials Faculty of Dentistry, Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences and Prosthodontics, School of Dentistry University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer","family":"El\u2010Fallal","sequence":"additional","affiliation":[{"name":"Department of Dental Biomaterials Faculty of Dentistry, Mansoura University  Mansoura Egypt"},{"name":"Department of Dental Biomaterials, Faculty of Oral and Dental Medicine Delta University for Science and Technology  Dakahlia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Badr","sequence":"additional","affiliation":[{"name":"Department of Dental Biomaterials, Faculty of Dentistry October 6 University  Cairo Egypt"},{"name":"Department of Dental Biomaterials, Faculty of Oral and Dental Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter X.","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences and Prosthodontics, School of Dentistry University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200802009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13286"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl9018935"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.07.002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.09.011"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevTherDrugCarrierSyst.2013006955"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.01.011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2014.12.001"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-022-01342-8"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201802852"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.04.018"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/12_2011_125"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2012.01.016"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1163\/092050611X564137"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.10.026"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4BM00161C"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/B406727D"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5PY00939A"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060319"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546503260757"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2017.08.011"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1928"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13534-011-0025-8"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41101241.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018914301328"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.133496"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/macp.200800093"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma4081384"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp980015p"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat998"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201801294"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(02)00008-1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.09.008"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1559-4106-8-19"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00127-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856296X00138"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2010.06.011"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30976"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp000113m"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2015.05.011"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201103056"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/la2001909"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.09.048"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201304995"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/am200204j"},{"key":"e_1_2_10_47_1","first-page":"706","article-title":"Electrospinning technology for nanofibrous scaffolds in tissue engineering","volume":"50","author":"Li W\u2010J","year":"2007","journal-title":"Nanotechnol Life Sci"},{"key":"e_1_2_10_48_1","article-title":"Fabrication and application of nanofibrous scaffolds in tissue engineering","volume":"42","author":"Li W\u2010J","year":"2009","journal-title":"Curr Protoc Cell Biol"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-018-0023-x"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10098"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6033"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.02.013"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20071694"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33021"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-011-9337-4"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-006-9001-5"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604508200"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218052109"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35546-6"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.2144\/000114065"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.06.021"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.06.003"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.37664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T09:08:47Z","timestamp":1727946527000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.37664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,8]]},"references-count":61,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1002\/jbm.a.37664"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.37664","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,8]]},"assertion":[{"value":"2023-05-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}