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This study aimed to assess the\u00a0potential of a peptide derived from cementum attachment protein (CAP\u2010p15) adsorbed onto aTiO<jats:sub>2<\/jats:sub> surfaces to promote the deposition of calcium phosphate (CaP) minerals and its impact on the adhesion and viability of human periodontal ligament cells (hPDLCs). <jats:styled-content style=\"fixed-case\">aTiO<jats:sub>2<\/jats:sub><\/jats:styled-content> surfaces were synthesized by magnetron sputtering technique. The <jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>\u2010p15 peptide was physically attached to <jats:styled-content style=\"fixed-case\">aTiO<jats:sub>2<\/jats:sub><\/jats:styled-content> surfaces and characterized by atomic force microscopy, fluorescence microscopy, and water contact angle measurement. We performed in vitro calcium phosphate nucleation assays using an artificial saliva solution (<jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\u20097.4) to simulate the oral environment. morphological and chemical characterization of the deposits were evaluated by scanning electronic microscopy (<jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content>) and spectroscopy molecular techniques (Raman Spectroscopy, <jats:styled-content style=\"fixed-case\">ATR\u2010FTIR<\/jats:styled-content>). The <jats:styled-content style=\"fixed-case\">aTiO<jats:sub>2<\/jats:sub><\/jats:styled-content> surfaces biofunctionalized with <jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>\u2010p15 were also analyzed for <jats:styled-content style=\"fixed-case\">hPDLCs<\/jats:styled-content> attachment, proliferation, and in vitro scratch\u2010healing assay. The results let us see that the homogeneous\u00a0amorphous titanium oxide coating was 70 nanometers thick. The CAP\u2010p15 (1\u2009\u03bcg\/mL) displayed the ability to adsorb onto the aTiO<jats:sub>2<\/jats:sub> surface, increasing the roughness and maintaining the hydrophilicity of the aTiO<jats:sub>2<\/jats:sub> surfaces. The physical adsorption of CAP\u2010p15 onto the aTiO<jats:sub>2<\/jats:sub> surfaces promoted the precipitation of a uniform layer of crystals with a flake\u2010like morphology and a Ca\/P ratio of 1.79. According to spectroscopy molecular analysis, these crystalline deposits correspond to carbonated hydroxyapatite. Regarding cell behavior, the biofunctionalized aTiO<jats:sub>2<\/jats:sub> surfaces improved the adhesion of hPDLCs after 24\u2009h of cell culture, achieving 3.4\u2010fold when compared to pristine surfaces. Moreover, there was an increase in cell proliferation and cell migration processes. Physical adsorption of CAP\u2010p15 onto aTiO<jats:sub>2<\/jats:sub> surfaces enhanced the formation of carbonate hydroxyapatite crystals and promoted the proliferation and migration of human periodontal ligament\u2010derived cells in in vitro studies. This experimental model using the novel bioactive peptide CAP\u2010p15 could be used as an alternative to increasing the osseointegration process of implants.<\/jats:p>","DOI":"10.1002\/jbm.a.37676","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T11:39:26Z","timestamp":1706528366000},"page":"1399-1411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of <scp>aTiO<sub>2<\/sub><\/scp> surfaces functionalized with <scp>CAP<\/scp>\u2010p15 peptide"],"prefix":"10.1002","volume":"112","author":[{"given":"Guadalupe","family":"Ureiro\u2010Cueto","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Periodontal y Tejidos Mineralizados, Divisi\u00f3n de Estudios de Posgrado e Investigaci\u00f3n Facultad de Odontolog\u00eda Universidad Nacional Aut\u00f3noma de  M\u00e9xico city 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