{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:45:32Z","timestamp":1780710332990,"version":"3.54.1"},"reference-count":77,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Corneal blindness affects over 10 million patients worldwide. Due to the limited supply of donor corneas and frequent graft failure, bioengineered alternatives are crucial. To overcome drawbacks associated with corneal substitutes from synthetic biomaterials, fabrication from plant\u2010derived biomaterials is a potential alternative. Herein, soy protein and glutenin in combination with different crosslinkers were evaluated for fabrication of corneal substitutes. Optical, mechanical, and biochemical properties of fabricated constructs and control rabbit corneas were evaluated <jats:italic>in vitro<\/jats:italic>. Soy protein crosslinked with peroxidase\/H<jats:sub>2<\/jats:sub>0<jats:sub>2<\/jats:sub> possessed transparency and mechanical properties comparable to controls, although their water content and biocompatibility were inferior. In contrast, soy protein crosslinked with tannic acid showed similar water content, tensile strength, and biocompatibility as rabbit corneas; however, these constructs displayed significantly lower transparency and higher strain to failure. Finally, glutenin cross\u2010linked using formaldehyde showed excellent transparency, strain to failure, and biocompatibility, however; they exhibited significantly lower water content and tensile strength than controls. This study is the first to establish CIELAB color values for the rabbit cornea, allowing quantitative optical evaluation of tissue\u2010engineered substitutes. Thus, a crosslinking strategy utilizing plant\u2010derived proteins for fabrication of constructs with properties comparable to rabbit corneas is a promising direction for development of tissue\u2010engineered corneal substitutes.<\/jats:p>","DOI":"10.1002\/jbm.a.37769","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T13:49:46Z","timestamp":1720100986000},"page":"2187-2201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of plant\u2010derived biomaterials for the development of tissue\u2010engineered corneal substitutes"],"prefix":"10.1002","volume":"112","author":[{"given":"Hadeel A. E.","family":"Badawy","sequence":"first","affiliation":[{"name":"Biochemistry Department, Faculty of Science Ain Shams University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Osman","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Faculty of Science Ain Shams University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6922-6193","authenticated-orcid":false,"given":"Tamer A. E.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute City of Scientific Research and Technology Applications (SRTA\u2010City)  Alexandria Egypt"},{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6134-5668","authenticated-orcid":false,"given":"Maxwell T.","family":"Hincke","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine University of Ottawa  Ottawa Ontario Canada"},{"name":"Department of Innovation in Medical Education (DIME), Faculty of Medicine University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e31816bdf54"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05\u20101339"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2012.04.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752\u20108062.2008.00083.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0721"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12265\u2010011\u20109301\u20103"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.244"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.30802\/AALAS\u2010JAALAS\u201020\u2010000016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3791\/66115"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u2010323\u201004835\u20104.50041\u20100"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102621.2006.tb15609.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0549"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jcrs.1998.0187"},{"issue":"4","key":"e_1_2_9_15_1","first-page":"311","article-title":"Extraction of unreduced glutenin from wheat flour with sodium dodecyl sulfate","volume":"58","author":"Danno G","year":"1981","journal-title":"Cereal Chem"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf034646x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0268\u2010005X(03)00128\u20100"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102621.1994.tb14709.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfoodeng.2003.10.013"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201013146\u20109"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010813742\u20100.00017\u20101"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.67.2.731"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811\u2010023\u201003000\u20104"},{"issue":"1","key":"e_1_2_9_25_1","first-page":"117","article-title":"An analysis of application of mutiple comparison test (post\u2010hoc) in Anova in recently published medical research literature","volume":"6","author":"Patel S","year":"2015","journal-title":"Nat J Commun Med"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasrep.2017.06.020"},{"key":"e_1_2_9_27_1","first-page":"108","volume-title":"Polymer Science: Research Advances, Practical Applications Educational Aspects","author":"Onofrei M","year":"2016"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.22203\/ecm.v005a01"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07\u20101348"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12132614"},{"key":"e_1_2_9_31_1","first-page":"1","volume-title":"Soy Protein\u2010Based Blends, Composites and Nanocomposites","author":"Visakh PM","year":"2017"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202100413"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43016\u2010020\u20100046\u20105"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986709787909613"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtadv.2022.100307"},{"issue":"4","key":"e_1_2_9_36_1","first-page":"29","article-title":"Heat exchanger analysis to reduce hexane loss in palm kernel oil extraction plant","volume":"13","author":"Sivaraos S","year":"2013","journal-title":"Int J Mech Mechatron Eng"},{"key":"e_1_2_9_37_1","first-page":"178","article-title":"Chemical composition of commercial soybean meals according to the origin of the beans","volume":"54","author":"C\u00e1mara L","year":"2017","journal-title":"Asociaci\u00f3n Espa\u00f1ola de Ciencia Av\u00edcola (WPSA Branch, AECA)"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.26021\/8342"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf800776b"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8BM01246F"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.10.067"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27238124"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073648"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JMSM.0000004002.11278.30"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbaa041"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.16.3.214.15412"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161\u20106420(01)00760\u20106"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41433\u2010022\u201002026\u20101"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1109\/TTHZ.2015.2392619"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym13030416"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2007.2670.2675"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.116.1.62"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05\u20101139"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.20381\/ruor\u201020003"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328213497413"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0965545X13050076"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748\u2010605x\/ac4d7b"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.01.020"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10570\u2010020\u201002999\u2010w"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11665\u2010020\u201004845\u20103"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.09.036"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2022.111636"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201016935\u2010w"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202104473"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1163\/092050610X532638"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i38.5973"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1493"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856\u2010015\u20105451\u20101"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u201094\u2010007\u20106262\u20106_6"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002\u2010221106"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.3923\/jms.2007.42.50"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20104827(61)90059\u20103"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328219899238"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110047"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0891\u20105849(96)00499\u20106"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727\u2010183\u201042413"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2007.0435"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439\u2010015\u20101280\u20100"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.37769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T05:10:41Z","timestamp":1728450641000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.37769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,4]]},"references-count":77,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/jbm.a.37769"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.37769","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,4]]},"assertion":[{"value":"2023-10-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}