{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:21:05Z","timestamp":1790619665159,"version":"4.1.0"},"reference-count":74,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T00:00:00Z","timestamp":1721001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1101902"],"award-info":[{"award-number":["2021YFA1101902"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0109100"],"award-info":[{"award-number":["2018YFA0109100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171107"],"award-info":[{"award-number":["32171107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    As the cornerstone of tissue engineering and regeneration medicine research, developing a cost\u2010effective and bionic extracellular matrix (ECM) that can precisely modulate cellular behavior and form functional tissue remains challenging. An artificial ECM combining polysaccharides and fibrillar proteins to mimic the structure and composition of natural ECM provides a promising solution for cardiac tissue regeneration. In this study, we developed a bionic hydrogel scaffold by combining a quaternized\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010chitin derivative (QC) and fibrin\u2010matrigel (FM) in different ratios to mimic a natural ECM. We evaluated the stiffness of those composite hydrogels with different mixing ratios and their effects on the growth of human umbilical vein endothelial cells (HUVECs). The optimal hydrogels, QCFM1 hydrogels were further applied to load HUVECs into nude mice for in vivo angiogenesis. Besides, we encapsulated human pluripotent stem cell\u2010derived cardiomyocytes (hPSC\u2010CMs) into QCFM hydrogels and employed 3D bioprinting to achieve batch fabrication of human\u2010engineered heart tissue (hEHT). Finally, the myocardial structure and electrophysiological function of hEHT were evaluated by immunofluorescence and optical mapping. Designed artificial ECM has a tunable modulus (220\u20131380\u2009Pa), which determines the different cellular behavior of HUVECs when encapsulated in these. QCFM1 composite hydrogels with optimal stiffness (800\u2009Pa) and porous architecture were finally identified, which could adapt for in vitro cell spreading and in vivo angiogenesis of HUVECs. Moreover, QCFM1 hydrogels were applied in 3D bioprinting successfully to achieve batch fabrication of both ring\u2010shaped and patch\u2010shaped hEHT. These QCFM1 hydrogels\u2010based hEHTs possess organized sarcomeres and advanced function characteristics comparable to reported hEHTs. The chitin\u2010derived hydrogels are first used for cardiac tissue engineering and achieve the batch fabrication of functionalized artificial myocardium. Specifically, these novel QCFM1 hydrogels provided a reliable and economical choice serving as ideal ECM for application in tissue engineering and regeneration medicine.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.37774","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T07:05:22Z","timestamp":1721027122000},"page":"2257-2272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Fabrication of chitin\u2010fibrin hydrogels to construct the 3D artificial extracellular matrix scaffold for vascular regeneration and cardiac tissue engineering"],"prefix":"10.1002","volume":"112","author":[{"given":"Pengcheng","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocatalysis and Enzyme Engineering, National &amp; Local Joint Engineering Research Center of High\u2010throughput Drug Screening Technology, School of Life Science Hubei University  Wuhan 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Center of High\u2010throughput Drug Screening Technology, School of Life Science Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8128-5874","authenticated-orcid":false,"given":"Donghui","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocatalysis and Enzyme Engineering, National &amp; Local Joint Engineering Research Center of High\u2010throughput Drug Screening Technology, School of Life Science Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Cai","sequence":"additional","affiliation":[{"name":"Hubei Engineering Center of Natural Polymers\u2010based Medical Materials, College of Chemistry and Molecular Sciences Wuhan University  Wuhan China"},{"name":"Institute of Hepatobiliary Diseases Zhongnan Hospital of Wuhan University  Wuhan 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