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In this study, the plasma\u2010based ion implantation (PBII) technique was performed on the surface of titanium alloy (Ti\u20106Al\u20104V, Ti64) using a mixture of nitrogen (N\n                    <jats:sub>2<\/jats:sub>\n                    ) and argon (Ar), resulting in a plasma\u2010nitrided surface (TiN\u2010Ti64). The surface composition of the TiN\u2010Ti64 was verified through X\u2010ray photoelectron spectroscopy (XPS). TiN\u2010Ti64 demonstrated superior hydrophilicity compared with Ti64. TiN\u2010Ti64 exhibited higher surface hardness than the original surface. The biological responses of primary human alveolar bone cells (hAVs) were observed on the TiN\u2010Ti64, revealing greater activation of cell adhesion and spreading compared with Ti64 and the control group (glass coverslip). Moreover, the TiN\u2010Ti64 significantly promoted cell proliferation compared with Ti64 and tissue culture plates. The mineralization of hAVs on the TiN\u2010Ti64 showed a significant increase, almost 20% greater than that of Ti64. Furthermore, a significant upregulation of mRNA expression for osteogenic differentiation marker genes, including\n                    <jats:italic>BMP2<\/jats:italic>\n                    ,\n                    <jats:italic>OCN<\/jats:italic>\n                    ,\n                    <jats:italic>OPN<\/jats:italic>\n                    , and\n                    <jats:italic>RUNX2<\/jats:italic>\n                    , was observed in TiN\u2010Ti64 compared with other conditions. In addition, the TiN\u2010Ti64 exhibited antibiofilm activity against\n                    <jats:italic>\n                      Streptococcus\n                      <jats:styled-content style=\"fixed-case\">aureus<\/jats:styled-content>\n                      .\n                    <\/jats:italic>\n                    In conclusion, the TiN\u2010Ti64, modified with the PBII technique utilizing a mixture of N\n                    <jats:sub>2<\/jats:sub>\n                    and Ar, emerges as a promising alternative for surface modification in dental implant applications.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.37853","type":"journal-article","created":{"date-parts":[[2024,12,22]],"date-time":"2024-12-22T11:49:05Z","timestamp":1734868145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nitrided Ti\u2010\n                    <scp>6Al<\/scp>\n                    \u2010\n                    <scp>4V<\/scp>\n                    : A Catalyst for Increase Mineralization and Osteogenic Marker Expression"],"prefix":"10.1002","volume":"113","author":[{"given":"Annop","family":"Krasaesin","sequence":"first","affiliation":[{"name":"Biomedical Engineering Institute Chiang Mai University  Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suttiporn","family":"Pinijsuwan","sequence":"additional","affiliation":[{"name":"School of Agro\u2010Industry Mae Fah Luang University  Chiang Rai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chatdanai","family":"Boonruang","sequence":"additional","affiliation":[{"name":"Center of Excellence in Materials Science and Technology, Department of Physics and Materials Science, Faculty of Science Chiang Mai University  Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanokwan","family":"Sriwattanapong","sequence":"additional","affiliation":[{"name":"Center 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