{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:22:44Z","timestamp":1791300164802,"version":"4.1.0"},"reference-count":99,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,2]],"date-time":"2025-02-02T00:00:00Z","timestamp":1738454400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002753","name":"Universidad Nacional de Colombia","doi-asserted-by":"publisher","award":["HERMES 51867"],"award-info":[{"award-number":["HERMES 51867"]}],"id":[{"id":"10.13039\/501100002753","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The activity of matrix metalloproteinases (MMPs) plays a crucial role in the aging of the resin\u2013dentin interface. The in\u00a0situ action of MMP\u20102 and MMP\u20109 has been confirmed in the process of dentin\u2010collagen degradation. However, the involvement of dental pulp cells in MMP secretion as a response to oxidative stress induced by contact with resin monomers has not been fully elucidated. Myricetin (MYR), like proanthocyanidin (PAC), has antioxidant properties and may help prevent extracellular matrix degradation. The objective was to evaluate the effect of MYR on the MMP expression and activity in response to reactive oxygen species (ROS) increase induced by triethylene glycol dimethacrylate (TEGDMA) in human odontoblast\u2013like cells (hOLCs). hOLCs differentiated from dental pulp mesenchymal stem cells were exposed to TEGDMA released from dentin blocks using a barrier model with transwell inserts for 18, 24, and 36\u2009h. Intracellular oxidation was evaluated using the 2\u2032,7\u2032\u2010dichlorofluorescein probe. The effect of 600\u2009\u03bcM MYR on the MMP\u20102 and MMP\u20109 expression was determined by reverse transcription\u2010quantitative polymerase chain reaction (RT\u2010qPCR). The extracellular MMP levels were quantified using enzyme\u2010linked immunosorbent assay, and their activation by means of a proteolytic fluorometric assay. The results were analyzed by one\u2010way analysis of variance and Tukey's post hoc test, <jats:italic>p\u2009\u2264<\/jats:italic>\u20090.05. TEGDMA exposure increased intracellular ROS and upregulated MMP\u20102 and MMP\u20109 mRNA in hOLCs (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). The levels of MMPs increased significantly 24\u2009h after TEGDMA exposure (<jats:italic>p<\/jats:italic>\u2009=\u20090.013). These secreted proteases exhibited high activation ability. MYR reduced ROS production and downregulated MMP expression and activity at both mRNA and protein levels, similar to the effect found for PAC, which was used as a control. A relationship was observed between MMP\u20102 and MMP\u20109 expression, secretion, and early activation with ROS increase due to TEGDMA exposure. MYR showed potential as a therapeutic strategy to control MMP expression and modulate redox imbalance, offering a protective effect on cellular response.<\/jats:p>","DOI":"10.1002\/jbm.a.37872","type":"journal-article","created":{"date-parts":[[2025,2,2]],"date-time":"2025-02-02T09:48:16Z","timestamp":1738489696000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Myricetin Modulates Matrix Metalloproteinases Expression Induced by <scp>TEGDMA<\/scp> in Human Odontoblast\u2013Like Cells"],"prefix":"10.1002","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3134-9198","authenticated-orcid":false,"given":"Paula Alejandra","family":"Baldi\u00f3n","sequence":"first","affiliation":[{"name":"Departamento de Salud Oral, Facultad de Odontolog\u00eda, Sede Bogot\u00e1 Universidad Nacional de Colombia  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6031-8015","authenticated-orcid":false,"given":"Carlos Aldemar","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Departamento de Salud Oral, Facultad de Odontolog\u00eda, Sede Bogot\u00e1 Universidad Nacional de Colombia  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9416-3841","authenticated-orcid":false,"given":"Diego Enrique","family":"Betancourt","sequence":"additional","affiliation":[{"name":"Departamento de Salud Oral, Facultad de Odontolog\u00eda, Sede Bogot\u00e1 Universidad Nacional de Colombia  Bogot\u00e1 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