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In the intervertebral disc (IVD), delivering growth factors faces challenges due to the antagonistic inflammatory environment and continuous mechanical stress, which can degrade biological agents and may reduce their local activity. To address this, we investigated the prolonged release of platelet\u2010derived growth factor isoforms BB (PDGF\u2010BB) and AB (PDGF\u2010AB) by using N\u2010desulfated heparin methacrylamide (Hep<jats:sup>\u2010N<\/jats:sup>) crosslinked within matrix\u2010metalloproteinase sensitive poly(ethylene glycol) (PEG) hydrogels. Using electrostatic interactions between the heparin derivative and PDGF, we optimized a sustained release dose of PDGF\u2010BB from the hydrogel in the presence of collagenase to mimic the in\u00a0vivo environment. We then assessed the effects of PDGF released from PEG\u2010hydrogel on human nucleus pulposus (NP) Cells. The MTT assay confirmed that 100 and 200\u2009ng doses significantly increased cell viability by 2.52\u2010fold and 2.46\u2010fold on Day 3, respectively. RT\u2010qPCR analysis revealed that PDGF\u2010AB and PDGF\u2010BB upregulated the expression of proliferation marker Ki\u201067 (MKI67) on both Day 3 and Day 5. Additionally, collagen type II alpha 1 chain (COL2A1) was significantly upregulated in the PDGF\u2010AB group on Day 5, indicating potential anabolic effects. These findings could pave the way for long\u2010term in\u00a0vivo studies on sustainable PDGF treatment for IVD degeneration.<\/jats:p>","DOI":"10.1002\/jbm.a.37918","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T10:20:22Z","timestamp":1746786022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>PDGF<\/scp>\u2010Releasing Hydrogels for Enhanced Proliferation of Human Nucleus Pulposus Cells"],"prefix":"10.1002","volume":"113","author":[{"given":"Takanori","family":"Fukunaga","sequence":"first","affiliation":[{"name":"Musculoskeletal Institute Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph J.","family":"Pearson","sequence":"additional","affiliation":[{"name":"Coulter Department of Biomedical Engineering Georgia Tech\/Emory University  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan Cree","family":"Miller","sequence":"additional","affiliation":[{"name":"Coulter Department of Biomedical Engineering Georgia Tech\/Emory University  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Musculoskeletal Institute Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Atlanta VA Medical Center  Decatur Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Lakrat","sequence":"additional","affiliation":[{"name":"Musculoskeletal Institute Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisbet","family":"Haglund","sequence":"additional","affiliation":[{"name":"Department of Surgery McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha Elena","family":"Diaz\u2010Hernandez","sequence":"additional","affiliation":[{"name":"Musculoskeletal Institute Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Atlanta VA Medical Center  Decatur Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9066-7954","authenticated-orcid":false,"given":"Johnna S.","family":"Temenoff","sequence":"additional","affiliation":[{"name":"Coulter Department of Biomedical Engineering Georgia Tech\/Emory University  Atlanta Georgia USA"},{"name":"Parker H. 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