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Therefore, there is a growing demand for new therapies that optimize drug bioavailability, allowing for lower therapeutic doses while enhancing cytocompatibility, maintaining antifungal, anti\u2010inflammatory, and wound healing efficacy. This study investigated the antifungal activity, cytocompatibility, wound healing potential, and mucosal adhesion of novel mucoadhesive formulations containing nystatin (NYS) or chlorhexidine (CHX) complexed with \u03b2\u2010cyclodextrin (\u03b2CD), compared with the drug\u2010free formulation (GEL) and the standard treatment with 2% miconazole gel (DK\u2014Daktarin). Efficacy against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Candida albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    was evaluated by measuring the metabolic activity, whereas cytocompatibility with human gingival fibroblasts (HGFs) was analyzed for viability, morphology, lactate dehydrogenase (LDH) release, and apoptosis. Additionally, wound healing potential was investigated by assessing cell migration efficacy, anti\u2010inflammatory activity, and reactive oxygen species (ROS) scavenging activity. Mucoadhesion was evaluated using mucin discs and a texture analyzer. Mucoadhesive gels containing \u03b2CD\u2010complexed NYS or CHX exhibited significantly higher antifungal activity when compared to the GEL and DK groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Compared to fibroblast control cultures, those exposed to drug\u2010complexed gels exhibited similar viability (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05) and morphological parameters, lower LDH release (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and similar apoptosis rates (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Additionally, exposure to the \u03b2CD\u2010modified gels was associated with complete wound closure (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), significant anti\u2010inflammatory effect, with downregulation of pro\u2010inflammatory gene expression (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and higher ROS scavenging activity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The developed formulations showed no difference in mucoadhesiveness (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), which was superior to that of DK (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Therefore, the proposed drug\u2010complexed mucoadhesives are promising therapeutic options for oral candidiasis.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.37990","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T10:41:19Z","timestamp":1758537679000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antifungal Activity, Cytocompatibility, and Wound Healing Potential of Novel Mucoadhesive Formulations for Oral Drug Delivery"],"prefix":"10.1002","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8934-4472","authenticated-orcid":false,"given":"Carolina Yoshi Campos","family":"Sugio","sequence":"first","affiliation":[{"name":"Department of Prosthodontics and Periodontics, Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo Brazil"},{"name":"Department of Removable Prosthodontics and Dental Materials, Dental School Federal University of Uberl\u00e2ndia (UFU)  Uberl\u00e2ndia Minas Gerais Brazil"},{"name":"BoneLab, Faculdade de Medicina Dent\u00e1ria Universidade do Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6910-1705","authenticated-orcid":false,"given":"Victor","family":"Martin","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Faculdade de Medicina Dent\u00e1ria Universidade do Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-2740","authenticated-orcid":false,"given":"L\u00eddia Maria Diogo","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy University of Lisbon  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4285-6646","authenticated-orcid":false,"given":"Priscileila Coleto","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences State University of Ponta Grossa (UEPG)  Ponta Grossa Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3320-8783","authenticated-orcid":false,"given":"Vanessa Migliorini","family":"Urban","sequence":"additional","affiliation":[{"name":"Department of Dentistry State University of Ponta Grossa (UEPG)  Ponta Grossa Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7086-2667","authenticated-orcid":false,"given":"Karin Hermana","family":"Neppelenbroek","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics, Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo 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