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Decellularized matrix (dECM) is a promising biomaterial; however, glycosaminoglycan (GAG) loss during decellularization limits its chondrogenic potential. In this study, we aimed to overcome this by developing a dECM hydrogel originating from cartilage, functionalized with the GAG chondroitin sulphate (CS), to replenish those originally depleted and incorporating quercetin to enhance hydrogel properties and chondrogenesis. An optimized decellularization protocol efficiently removed DNA, but with a significant loss of GAGs (73%). After dECM solubilization, functionalization with CS or aldehyde modified CS (mCS) was performed. CS\u2010functionalized hydrogels maintained low stiffness compared to non\u2010functionalized hydrogel, while 0.2\u2009mg\/mL mCS hydrogels exhibited significantly slower gelation kinetics. To aid the hydrogel's chondrogenic ability, a novel approach using quercetin was investigated. Incorporation of 0.3\u2009mg\/mL quercetin in 0.4\u2009mg\/mL mCS\u2010functionalized hydrogels resulted in increased gel stiffness. The impact on cell viability and chondrogenic differentiation was evaluated. Results showed similar cell viability in dECM and CS\u2010functionalized hydrogels at 1 and 3\u2009days of culture, with no significant changes in gene expression of chondrogenic and hypertrophic genes. In quercetin\u2010containing hydrogels, the viability of human dermal fibroblasts was not significantly different from non\u2010functionalized hydrogels, while human chondrocytes showed a significant upregulation of collagen type II, with 6.6\u2010 and 2.2\u2010fold increases for 0.15 and 0.3\u2009mg\/mL quercetin, respectively. These results provide an initial proof\u2010of\u2010concept for dECM functionalization strategies that restore lost CS while incorporating quercetin, creating a microenvironment favorable for cartilage repair.<\/jats:p>","DOI":"10.1002\/jbm.a.37999","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T05:57:26Z","timestamp":1759471046000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Decellularised Cartilage\u2010Based Hydrogels Functionalised With Chondroitin Sulphate and Quercetin: The Impact on Chondrogenesis"],"prefix":"10.1002","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1215-693X","authenticated-orcid":false,"given":"Nuno Da Silva","family":"Rosa","sequence":"first","affiliation":[{"name":"i3S\u2014Instituto de Inova\u00e7\u00e3o e Investiga\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Porto Portugal"},{"name":"INEB\u2014Instituto de Engenharia 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