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This underscores the need to develop a three\u2010dimensional porous microencapsulation scaffold system that can replicate a supportive microenvironment, providing both mechanical stability and biological cues vital to preserving islet viability and function. This study investigated the potential of two freeze\u2010dried and crosslinked gelatin\u2010based scaffolds: Gelatin vinyl acetate copolymer (GeVAc) and gelatin with oxidized dextran dialdehyde (GELDEX), for supporting pancreatic islet culture. Their physicochemical properties, architecture, and wettability were analyzed using scanning electron microscopy and contact angle measurements. Both scaffolds exhibited hydrophilic, biocompatible, and structurally stable characteristics. Mouse pancreatic MIN6 cells were cultured on the scaffolds for 7\u2009days to evaluate islet viability, extracellular matrix deposition and functionality through immunocytochemistry, glucose\u2010stimulated insulin secretion (GSIS), and gene expression analysis. MIN6 cells adhered well to both scaffolds, forming dense monolayers and multicellular spheroids that resembled native islet clusters. GeVAc scaffolds showed significantly higher glucose sensitivity and glucose stimulation index (GSI) compared to GELDEX. While INS1 and PDX1 expression levels were comparable in both scaffolds, NKX6.1 expression was significantly higher in GeVAc. These findings indicate that scaffold architecture and surface characteristics play a crucial role in creating a supportive microenvironment for islet cluster formation, highlighting the potential of gelatin\u2010based scaffolds as microencapsulation platforms for clinical islet transplantation in diabetes therapy.<\/jats:p>","DOI":"10.1002\/jbm.a.70079","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T06:21:43Z","timestamp":1776234103000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biomimetic Gelatin\u2010Based\n                    <scp>3D<\/scp>\n                    Scaffolds for Enhanced Islet Microencapsulation and Functionality in Diabetes Therapy"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6880-4166","authenticated-orcid":false,"given":"Rukhiya","family":"Salim","sequence":"first","affiliation":[{"name":"Division of Tissue Engineering and Regeneration Technologies, Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology  Trivandrum Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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