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With rising antibiotic resistance, bioactive glasses such as F18 and 45S5 have emerged as potential alternative antimicrobial agents. To evaluate the antimicrobial effects of F18 and 45S5 on\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolates from CRS patients, focusing on planktonic growth, biofilm formation, and mechanisms of action. Clinical\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolates were exposed to F18 and 45S5 (0\u2013512\u2009\u03bcg\/mL) using a modified Calgary Biofilm Device. Biofilm biomass was quantified by crystal violet staining and spectrophotometry. A pH\u2010controlled assay was performed to determine whether alkalinization mediated antibiofilm effects. Inductively coupled plasma analysis characterized F18 ion\u2010release kinetics. Expression of biofilm\u2010related genes (icaA, icaB, icaD, agrA, agrC) was quantified, and biofilm morphology was examined by scanning electron microscopy. Both bioglasses inhibited biofilm formation at concentrations \u2265\u2009128\u2009\u03bcg\/mL but did not eradicate mature biofilms. At 512\u2009\u03bcg\/mL, F18 and 45S5 reduced the biofilm optical density by 78% and 67%, respectively. Although pH elevation occurred, biofilm inhibition was independent of pH variation. F18 exposure downregulated ica operon genes but not agr genes. Ion analysis demonstrated concentration\u2010dependent release of Ca and Si, with no evidence of apatite formation. SEM showed reduced extracellular matrix and altered bacterial organization. F18 and 45S5 inhibit\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    biofilm formation through pH\u2010independent mechanisms associated with ion release and suppression of matrix\u2010related genes, supporting their potential as adjunctive strategies in CRS management.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.70082","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:49:07Z","timestamp":1778842147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Antibiofilm Approach Using Bioactive Glasses\n                    <scp>F18<\/scp>\n                    and\n                    <scp>45S5<\/scp>\n                    Against Clinical Isolates of\n                    <i>Staphylococcus aureus<\/i>\n                    in Chronic Rhinosinusitis"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1554-2489","authenticated-orcid":false,"given":"Jo\u00e3o Vitor Bizinoto","family":"Caetano","sequence":"first","affiliation":[{"name":"Division of Otorhinolaryngology, Ribeirao Preto School of Medicine University of Sao Paulo (FMRP\u2010USP)  Ribeirao Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2902-5952","authenticated-orcid":false,"given":"Maria Clara Antunes","family":"Alves","sequence":"additional","affiliation":[{"name":"Division of Otorhinolaryngology, Ribeirao Preto School of Medicine University of Sao Paulo (FMRP\u2010USP)  Ribeirao Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5259-7513","authenticated-orcid":false,"given":"Marina Zilio","family":"Fantucci","sequence":"additional","affiliation":[{"name":"Division of Otorhinolaryngology, Ribeirao Preto School of Medicine University of Sao Paulo (FMRP\u2010USP)  Ribeirao Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1673-264X","authenticated-orcid":false,"given":"Lilian Eslaine Costa Mendes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Division of Otorhinolaryngology, Ribeirao Preto School of Medicine University of Sao Paulo (FMRP\u2010USP)  Ribeirao Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9123-2728","authenticated-orcid":false,"given":"Denny Marcos","family":"Garcia","sequence":"additional","affiliation":[{"name":"Division of Otorhinolaryngology, Ribeirao Preto School of Medicine University of Sao Paulo (FMRP\u2010USP)  Ribeirao Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0291-6034","authenticated-orcid":false,"given":"Marina Trevelin","family":"Souza","sequence":"additional","affiliation":[{"name":"Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering Federal University of S\u00e3o Carlos  S\u00e3o Carlos Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2492-3324","authenticated-orcid":false,"given":"Rafael Soares","family":"Stenico","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Philosophy, Sciences, and Letters at Ribeirao Preto University of Sao Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2320-3348","authenticated-orcid":false,"given":"M\u00e1rcia Andreia Mesquita Silva","family":"da Veiga","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Philosophy, Sciences, and Letters at Ribeirao Preto University of Sao Paulo  Ribeir\u00e3o Preto 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