{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T18:01:23Z","timestamp":1785002483796,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Delivering a persistent biopolymer matrix to the superficial dermis remains a significant challenge for microneedle systems due to the inherent limitations of conventional dissolving platforms. Here, we present a detachable microneedle design that permits rapid needle separation during administration. To biologically compensate for the relatively short 350\u2009\u03bcm needle geometry designed for at\u2010home safety, this system utilizes a crosslinked hyaluronic acid (cHA) matrix loaded with cosmetic peptides and functional excipients. The primary objective is to reliably deliver this composite biochemical cHA matrix into the skin to a depth of ~300\u2009\u03bcm, which corresponds to the dermal\u2013epidermal junction. Segmented mechanical compression analysis revealed that the non\u2010polymeric payload did not compromise the matrix; rather, it significantly improved the initial axial stiffness from 0.059\u2009\u00b1\u20090.004 to 0.081\u2009\u00b1\u20090.005\u2009N\/mm per needle. This structural enhancement minimized axial displacement under load, enabling the 350\u2009\u03bcm needles to reliably reach an ex\u00a0vivo penetration depth of 335\u2009\u00b1\u200922\u2009\u03bcm. A 14\u2010day clinical evaluation (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200928) using three\u2010dimensional topography demonstrated excellent clinical tolerability with no adverse effects and significant reductions in surface roughness (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>a<\/jats:italic>\n                    <\/jats:sub>\n                    ) and maximum wrinkle height (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>t<\/jats:italic>\n                    <\/jats:sub>\n                    ) both immediately and at the conclusion of treatment.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.70113","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T13:19:00Z","timestamp":1782911940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioactive\u2010Loaded Detachable Crosslinked Hyaluronic Acid Microneedles: Structural Validation and Clinical Anti\u2010Wrinkle Efficacy"],"prefix":"10.1002","volume":"114","author":[{"given":"Puttikorn","family":"Pukfukdee","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science Silpakorn University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supanan","family":"Ampawa","sequence":"additional","affiliation":[{"name":"Mineed Technology Co. Ltd.  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirada","family":"Littirak","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Silpakorn University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supakij","family":"Suttiruengwong","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, Sustainable Materials Laboratory, Faculty of Engineering and Industrial Technology Silpakorn University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pravit","family":"Asawanonda","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Dermatology, Faculty of Medicine Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supason","family":"Wanichwecharungruang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Center of Excellence in Materials and Bio\u2010Interfaces, Faculty of Science Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/derm.22028"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1297637"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2016.09.015"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a015370"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00020.2004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04925"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.13426"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e318188245e"},{"issue":"7","key":"e_1_2_9_11_1","first-page":"36","article-title":"Microneedling Therapy for Atrophic Acne Scars: An Objective Evaluation","volume":"8","author":"El\u2010Domyati M.","year":"2015","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"issue":"3","key":"e_1_2_9_12_1","article-title":"Physiological Mechanisms and Therapeutic Applications of Microneedling: A Narrative Review","volume":"17","author":"Tehrani L.","year":"2025","journal-title":"Cureus"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/jdds.jdds_126_20"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2010.01778.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/asj\/sjv050"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40486-024-00204-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmaterialsau.4c00125"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202401782"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37933"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.0c00498"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1467-2494.2002.00153.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom15010088"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cosmetics2030236"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.04.010"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2003.12.010"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/dsu.12301"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27154956"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2019.03.014"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1411.11029"},{"issue":"2","key":"e_1_2_9_30_1","first-page":"37","article-title":"Double\u2010Blind, Randomized Trial on the Effectiveness of Acetylhexapeptide\u20103 Cream and Palmitoyl Pentapeptide\u20104 Cream for Crow's Feet","volume":"16","author":"Aruan R. 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