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In this study, an efficient nanofibrous core\u2010shell biomaterial was fabricated for the restoration of damaged tissue using a polycaprolactone\/polyvinyl alcohol\u2010based emulsion loaded with \u03b2\u2010carotene and keratin hydrolysate (KH) by electrospinning. The fabricated nanofiber was examined for its morphological, physicochemical, thermal, and mechanical properties. An in\u00a0vitro drug release study was performed to reveal the release profiles of incorporated \u03b2\u2010carotene and KH. The antioxidant capacity of the nanofiber was measured by DPPH and ABTS assays. The effect of nanofibers on cell morphology, proliferation, migration, adhesion, and angiogenic potential was examined using fibroblast (NIH\/3\u2009T3) and endothelial (EA.hy926) cells. The results showed that the emulsion electrospun fiber loaded with \u03b2\u2010carotene and KH had a diameter of 249\u2009nm, and the fibrous mat had a contact angle of 39.92\u00b0. It exhibited tensile strength of 2.05\u2009MPa and an elongation at break value of 23.55%. A prolonged release of \u03b2\u2010carotene and a burst release of KH from the nanofiber were noticed in the drug release study. Interestingly, nanofibers incorporated with \u03b2\u2010carotene and KH exerted improved cell proliferation, migration, cell adhesion, and tube formation. It supported the expression of genes associated with angiogenic signaling in both fibroblast and endothelial cells. In conclusion, this study highlights the potential of electrospun nanofibers derived from emulsions for tissue regeneration, and it could be employed for accelerated wound repair when infused with \u03b2\u2010carotene and KH.<\/jats:p>","DOI":"10.1002\/jbm.a.70120","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T08:21:03Z","timestamp":1783153263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cellular Insights Into Proangiogenic Activation in Fibroblast and Endothelial Cells by Dual Drug\u2010Loaded Emulsion Electrospun Nanofibers for Enhanced Tissue Regeneration"],"prefix":"10.1002","volume":"114","author":[{"given":"Vinayagamurthy","family":"Kalaiselvi","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babu","family":"Liya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalavarthi","family":"Karthika","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumar Deena","family":"Praveena","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen Nimish","family":"Mol","sequence":"additional","affiliation":[{"name":"Department of Fish Processing Technology Dr MGR Fisheries College and Research Institute, Dr. J Jayalalithaa Fisheries University  Ponneri India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anandasadagopan Suresh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niraikulam","family":"Ayyadurai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Numbi Ramudu","family":"Kamini","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory and CARE (Centre for Academic and Research Excellence) Division, CSIR \u2010 Central Leather Research Institute  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4723-1765","authenticated-orcid":false,"given":"Ponesakki","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology CSIR\u2010Central Leather Research Institute  Chennai India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3406215"},{"key":"e_1_2_11_3_1","first-page":"6","article-title":"Physiology of Wound Healing","volume":"13","author":"Hunt T. 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