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The nanoparticles (NCBRPE), produced by nanoprecipitation, were evaluated for size, polydispersity index (PdI), zeta potential, and encapsulation efficiency (EE). Stability assessments over 365\u2009days confirmed preserved physicochemical properties. Release kinetics, evaluated using a dialysis membrane assay and UPLC\u2010MS, revealed that vestitol and formononetin followed the Peppas\u2010Korsmeyer model, while biochanin A followed Higuchi kinetics, indicating diffusion\u2010controlled mechanisms. Flow cytometry and confocal microscopy showed lower uptake of NCBRPE compared to free BRPE, though uptake was significantly higher in OVCAR\u20103 cells, suggesting receptor\u2010mediated internalization. Cell cycle analysis revealed that NCBRPE, but not BRPE, induced a shift from G1 to S phase, potentially impairing cell proliferation. Migration assays demonstrated significant inhibition of OVCAR\u20103 cell migration by both BRPE and NCBRPE. Acute toxicity evaluation in\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    confirmed the safety of NCBRPE, with over 80% survival at doses up to 50\u2009mg\/kg. These results demonstrate that nanoencapsulation enhances the stability, release control, and therapeutic activity of BRPE, supporting its potential as a multifunctional nanoplatform for treating female cancers, particularly ovarian carcinoma.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.70124","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:58:30Z","timestamp":1788519510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Brazilian Red Propolis Nanoparticles: In\u00a0Vitro Anticancer Effects in Female\u2010Associated Malignancies and In\u00a0Vivo Toxicity in an Alternative Model"],"prefix":"10.1002","volume":"114","author":[{"given":"Isabela A.","family":"Justino","sequence":"first","affiliation":[{"name":"GNanoBio, School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo  Ribeirao Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline E. 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