{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:02:41Z","timestamp":1789459361061,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In this exploratory pilot study, we evaluated bioluminescent trace labeling as a strategy for monitoring the in\u00a0vivo distribution of cross\u2010linked porous protein microcrystals grown from the CJ protein. CJ crystals doped with CJ\u2010SpyTag003 were functionalized with SpyCatcher003\u2010NanoLuciferase and imaged after intravenous administration in BALB\/c mice. In\u00a0vitro experiments confirmed that SpyCatcher\u2010fusion proteins could be retained by CJ\u2010SpyTag003 crystals and that NanoLuciferase remained active after association with the crystal scaffold. Following tail\u2010vein injection, whole\u2010animal IVIS imaging revealed early thoracic signal consistent with first\u2010pass pulmonary exposure, followed by rapid signal decay. A subset of animals showed persistent hindlimb\u2010associated signal, but terminal ex\u00a0vivo imaging at 72\u2009h showed no statistically significant organ\u2010level differences after sensitivity analysis excluding one extreme muscle\u2010associated value. These results demonstrate the feasibility of NanoLuciferase\u2010based trace labeling for longitudinal imaging of CJ microcrystals while highlighting important interpretive limitations of substrate\u2010dependent bioluminescence, including furimazine delivery, local substrate pooling, reporter stability, and the inability of bioluminescence alone to distinguish intact crystals from released or surface\u2010associated NanoLuciferase. Overall, this work provides an initial in\u00a0vivo imaging framework for CJ protein microcrystals and identifies particle size, reporter stability, and orthogonal tracking as priorities for future pharmacokinetic studies.<\/jats:p>","DOI":"10.1002\/jbm.a.70127","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:07:43Z","timestamp":1788944863000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioluminescent Trace Labeling of Porous Protein Microcrystals for Evaluating Their Pharmacodynamics"],"prefix":"10.1002","volume":"114","author":[{"given":"Alec","family":"Jones","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anika","family":"O'Brian","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering 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