{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:29:00Z","timestamp":1786699740366,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172427"],"award-info":[{"award-number":["82172427"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Currently, there is a lack of effective methods to repair annulus fibrosus (AF) defects in clinical practice. Therefore, exploring strategies to promote AF regeneration is of significant importance for delaying intervertebral disc degeneration. In recent years, tissue engineering technology has become a hotspot in the field of AF repair, offering promising treatments for intervertebral disc degenerative diseases. Studies have shown that surface structural characteristics, such as the fiber orientation of scaffold materials, play a crucial role in regulating the differentiation of cells adhering to the scaffold surface. Here we used electrospinning technology to prepare aligned and random decellularized annulus fibrosus matrix (DAFM)\/poly(ether carbonate urethane)urea (PECUU) blended fibrous scaffolds. Rat AF stem cells (AFSCs) cultured on the aligned DAFM\/PECUU blended fibrous scaffolds exhibited an aligned distribution, and the secreted AF\u2010associated extracellular matrix components, including\n                    <jats:italic>Collagen type I<\/jats:italic>\n                    ,\n                    <jats:italic>Collagen type II<\/jats:italic>\n                    , and\n                    <jats:italic>Aggrecan<\/jats:italic>\n                    , were also aligned. In contrast, AFSCs on the random DAFM\/PECUU blended fibrous scaffolds exhibited an irregular distribution, and the secreted AF\u2010associated extracellular matrix components were also irregularly distributed. Moreover, AFSCs on the aligned DAFM\/PECUU blended fibrous scaffolds expressed higher levels of\n                    <jats:italic>Col\u2010I<\/jats:italic>\n                    and\n                    <jats:italic>Aggrecan<\/jats:italic>\n                    than those on the random DAFM\/PECUU blended fibrous scaffold. Both aligned and random DAFM\/PECUU blended fibrous scaffolds degraded slowly in\u00a0vivo. Magnetic resonance imaging, hematoxylin and eosin staining, Safranin O\u2010fast green staining, and immunohistochemical staining demonstrated that both aligned and random DAFM\/PECUU blended fibrous scaffolds effectively repaired AF defects in rats in\u00a0vivo. However, the aligned DAFM\/PECUU blended fibrous scaffolds exhibited superior repair capability.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.70140","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T06:28:43Z","timestamp":1786343323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Reparative Ability of the Aligned Decellularized Annulus Fibrosus Matrix\/Poly(Ether Carbonate Urethane)Urea Blended Fibrous Scaffolds on Annulus Fibrosus Defects"],"prefix":"10.1002","volume":"114","author":[{"given":"Yifeng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Spine Surgery Yijishan Hospital of Wannan Medical College  Wuhu Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery Yijishan Hospital of Wannan Medical College  Wuhu Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8924-0582","authenticated-orcid":false,"given":"Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery Yijishan Hospital of Wannan Medical College  Wuhu Anhui China"},{"name":"Anhui Province Key Laboratory of Non\u2010Coding RNA Basic and Clinical Transformation  Wuhu Anhui 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