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Here, we developed a structurally defined 3D\u2010printed ordered porous chitosan methacrylate\/gelatin methacryloyl (CSMA\/GelMA) hydrogel scaffold platform to test whether topological order acts as a structurally relevant biomaterial cue for adipose\u2010derived mesenchymal stem cell (ADSC) regulation. To enrich for topology\u2010dependent effects, ordered and disordered scaffolds were fabricated from the same photocrosslinkable chitosan methacrylate\/gelatin methacryloyl precursor system and subjected to the same crosslinking and lyophilization\u2013rehydration workflow, while differing in spatial pore organization. Among the ordered architectures tested, the 200\u2009\u03bcm scaffold showed the most favorable early biological performance and was selected for subsequent studies. Ordered topology induced ADSC polarization, directional F\u2010actin organization, and nuclear elongation\/alignment, indicating coordinated cytoskeletal\u2013nuclear remodeling. Mechanistically, ordered scaffolds increased FAK phosphorylation and enhanced YAP nuclear localization, whereas pharmacological inhibition of FAK attenuated YAP\u2010related responses and the reparative paracrine advantage conferred by ordered topology. On this basis, sustained SDF\u20101\u03b1 presentation further enhanced the reparative secretory profile, at least partly through CXCR4\u2010AKT\/ERK\u2010associated signaling. In an STZ\u2010induced diabetic full\u2010thickness skin wound model, the integrated ordered scaffold strategy improved wound closure, histological reconstruction, collagen deposition, and CD31\u2010 and CD206\u2010associated reparative changes, with the greatest benefit observed when combined with ADSCs. These findings support a topology\u2013mechanotransduction\u2013function axis within this scaffold system and provide a rationale for biomaterial\u2010guided stem\u2010cell therapy in diabetic wound healing.<\/jats:p>","DOI":"10.1002\/jbm.a.70145","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:21:01Z","timestamp":1789024861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>3D<\/scp>\n                    \u2010Printed Ordered Porous\n                    <scp>CSMA<\/scp>\n                    \/\n                    <scp>GelMA<\/scp>\n                    Hydrogel Scaffolds Enhance\n                    <scp>ADSC<\/scp>\n                    Reparative Function With\n                    <scp>FAK<\/scp>\n                    \u2013\n                    <scp>YAP<\/scp>\n                    \u2010Related Mechanotransductive Changes"],"prefix":"10.1002","volume":"114","author":[{"given":"Songlu","family":"Tseng","sequence":"first","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"},{"name":"Center for Regenerative Medicine &amp; Plastic Surgery Research Peking Union Medical College Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieyu","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"},{"name":"Center for Regenerative Medicine &amp; Plastic Surgery Research Peking Union Medical College Hospital  Beijing 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