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Here, we established an integrated lectin\u2010based workflow to profile the glycan landscape of commercially sourced bovine pepsin\u2010soluble Col\u2010I as supplied for biomaterial use. Using a panel of 15 biotinylated and fluorescent lectins, we combined qualitative fluorescence staining, quantitative enzyme\u2010linked lectin sorbent assay (ELLSA), and lectin\u2010probed western blotting to compare three production batches. The batches shared common mannose\u2010 and galactose\u2010reactive signals but displayed marked heterogeneity in low\u2010abundance fucosylated and sialylated epitopes. Haptenic sugar inhibition and targeted digestion with \u03b1(1,6)\u2010fucosidase and \u03b1(2,3)\u2010neuraminidase confirmed lectin specificity and showed that some lectin\u2010reactive motifs were partially masked in native films. In light of the known restriction of classical Type I collagen glycosylation to galactosyl\u2010 and glucosylgalactosyl\u2010hydroxylysine, together with physicochemical evidence consistent with residual non\u2010collagenous components, the complex fucosylated and sialylated signals detected here most likely arise predominantly from co\u2010purified collagen\u2010associated extracellular\u2010matrix glycoproteins rather than from the collagen \u03b1\u2010chains alone. Functionally, all Col\u2010I\u2010coated surfaces strongly promoted MDA\u2010MB\u2010231 cell adhesion, while glycosidase treatment produced batch\u2010dependent visual trends in wound\u2010closure kinetics without significant differences at matched time points. Overall, this work identifies a hidden source of batch variability in commercial collagen biomaterials and positions lectin profiling as an accessible quality\u2010control tool for characterizing the full glycoprotein composition of collagen raw materials.<\/jats:p>","DOI":"10.1002\/jbm.a.70148","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:59:47Z","timestamp":1788519587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Profiling the Glycosignature of Commercial Pepsin\u2010Soluble Type I Collagen Preparations: A MultiModal Lectin Strategy Reveals Latent Batch Variability"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4569-0194","authenticated-orcid":false,"given":"Mohamed","family":"EL\u2010Melegy","sequence":"first","affiliation":[{"name":"RINN Medical Devices University of Galway  Galway 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