{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:53:05Z","timestamp":1792162385030,"version":"4.4.0"},"reference-count":75,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007246","name":"Y\u00fcksek\u00f6\u011fretim Kurulu","doi-asserted-by":"publisher","award":["Y\u00d6K 100\/2000 Priority Areas Program"],"award-info":[{"award-number":["Y\u00d6K 100\/2000 Priority Areas Program"]}],"id":[{"id":"10.13039\/501100007246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Calcium phosphates alone have a limited induction potential in challenging bone conditions, necessitating strategies that enhance cellular responses and matrix\u2010related processes. An injectable formulation combining calcium\u2010deficient hydroxyapatite (CDHA) with mesenchymal stem cell\u2010derived extracellular vesicles may present a promising approach for modulating osteogenic activity. A dual formulation composed of calcium\u2010deficient HA and human bone marrow mesenchymal stem cell\u2010derived extracellular vesicles, incorporating Annexin V as a binding molecule, was generated and validated. Structural and molecular characterization was performed using Raman spectroscopy, electron paramagnetic resonance, and sodium dodecyl\u2010sulfate polyacrylamide gel electrophoresis (SDS\u2010PAGE). Real\u2010time impedance\u2010based cell proliferation analysis was applied to determine biologically effective concentrations. The effects of the formulation on human osteoblast behavior and early osteogenic activity were additionally evaluated. Raman spectroscopy confirmed structural integration within the formulation termed CHAnEx, showing peak shifts and spectral features at 959, 630, and 806\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    , indicating molecular interactions between calcium\u2010deficient HA, Annexin V, and extracellular vesicles. Electron paramagnetic resonance (EPR) analysis revealed distinct g\u2010factor shifts consistent with binding interactions. Real\u2010time proliferation analysis identified biologically effective concentrations of calcium\u2010deficient HA and extracellular vesicles using human osteoblast models. The combined formulation increased alkaline phosphatase activity, showing approximately a 20%\u201325% increase compared to control groups. These findings indicate that the calcium\u2010deficient HA\u2010extracellular vesicle formulation supports functional interactions and modulates osteoblast responses under in\u00a0vitro conditions, suggesting its relevance as an injectable biomaterial platform.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.70159","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:11:17Z","timestamp":1789647077000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Annexin V\u2010Functionalized Injectable Calcium\u2010Deficient Hydroxyapatite for Extracellular Vesicles Binding and Controlled Release"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6900-4702","authenticated-orcid":false,"given":"Eda","family":"Ciftci","sequence":"first","affiliation":[{"name":"Department of Bioengineering Graduate School of Science and Engineering, Hacettepe University, 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