{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:53:07Z","timestamp":1792162387815,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL143008"],"award-info":[{"award-number":["HL143008"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL170573"],"award-info":[{"award-number":["HL170573"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL163085"],"award-info":[{"award-number":["HL163085"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5F31HL168909\u201003"],"award-info":[{"award-number":["5F31HL168909\u201003"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Bioprosthetic heart valves (BHV), typically fabricated from glutaraldehyde\u2010fixed bovine pericardium (BP), are the most common type of replacement for patients with severe heart valve disease. Despite being preferred, BHV are limited in durability due to structural valve degeneration (SVD), which occurs due to leaflet calcification and non\u2010calcific mechanisms including advanced glycation end product (AGE) accumulation and serum protein infiltration. Another limitation of BHV is characteristically they do not endothelialize, even after years of implantation. Previously we showed pretreating BP with Pyridoxamine (PYR), a vitamer of vitamin B6, effectively mitigated AGE accumulation in\u00a0vitro and in\u00a0vivo. The present studies investigated the effect of PYR pretreated BHV on endothelial cell\u2010material interactions, using bone marrow origin human blood outgrowth endothelial cells (BOEC) as a model cell type; the hypothesis was PYR pretreatment would provide a more favorable surface for endothelialization compared to glut\u2010fixed BP. Static studies compared untreated BP versus PYR\u2010BP to examine the effect on cell adhesion; PYR\u2010BP had significantly more BOEC adhered than untreated BP measured by Alamar blue assays. PYR pretreatment under non\u2010AGE and model AGE conditions were also investigated via static and high shear studies. PYR pretreated collagen coated\u2010glutaraldehyde fixed slides significantly increased BOEC attachment over time under AGE and non\u2010AGE conditions. Under arterial shear levels, PYR pretreatment increased BOEC retention compared to untreated slides under non\u2010AGE and AGE conditions. In conclusion, PYR pretreated BP or model, thin\u2010layer collagen surfaces promote endothelial cell attachment and reduced cytotoxicity under static and shear conditions, with or without AGE presence.<\/jats:p>","DOI":"10.1002\/jbm.a.70175","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:51:14Z","timestamp":1791874274000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vitamin\n                    <scp>B6<\/scp>\n                    Modification of Bovine Pericardial Bioprosthetic Heart Valve Leaflets Promotes Blood Outgrowth Endothelial Cell Adhesion"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6449-6226","authenticated-orcid":false,"given":"Tina E.","family":"Thomas","sequence":"first","affiliation":[{"name":"Department of Bioengineering, School of Engineering and Applied Science The University of Pennsylvania  Philadelphia 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2311-4728","authenticated-orcid":false,"given":"Alexis","family":"Martinez","sequence":"additional","affiliation":[{"name":"The Topolewski Pediatric Heart Valve Center, Division of Cardiology, Department of Pediatrics The Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9233-1752","authenticated-orcid":false,"given":"Stanley J.","family":"Stachelek","sequence":"additional","affiliation":[{"name":"The Topolewski Pediatric Heart Valve Center, Division of Cardiology, Department of Pediatrics The Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"},{"name":"Perelman School of Medicine, The University of Pennsylvania  Philadelphia Pennsylvania 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