{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:48:53Z","timestamp":1761918533972,"version":"3.37.3"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,10,16]],"date-time":"2003-10-16T00:00:00Z","timestamp":1066262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Conacyt (M\u00e9xico);","award":["28893-M"],"award-info":[{"award-number":["28893-M"]}]},{"name":"FOFOI- IMSS","award":["SP0038\/1289"],"award-info":[{"award-number":["SP0038\/1289"]}]},{"name":"CONACYT-CSIC","award":["E130-882"],"award-info":[{"award-number":["E130-882"]}]},{"DOI":"10.13039\/100011999","name":"FUNSALUD","doi-asserted-by":"crossref","award":["101-001-003"],"award-info":[{"award-number":["101-001-003"]}],"id":[{"id":"10.13039\/100011999","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2003,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biodegradable nerve guides have represented new treatment alternatives for nerve repairing. They are gradually biodegradable, exert biological effects directly to the injured nerve, and act as drug\u2010 or cell\u2010delivery devices. Furthermore, progesterone (PROG) has been demonstrated to promote injured peripheral nerve regeneration. In this study, it was hypothesized that PROG delivered from chitosan prostheses provides better facial nerve regenerative response than chitosan prostheses with no PROG. As there are no reports on the use of the former as nerve\u2010guide material in the regeneration of injured nerves, this is the main objective of the present work. Chitosan prostheses containing PROG were used to bridge 10\u2010mm gaps in rabbit facial nerves. The regenerated nerves were evaluated 45 days after implantation in animals with the use of light microscopy and morphometric analysis. Gas chromatography was used in order to quantify PROG content in prosthesis prior to and after implantation in subcutaneous tissue at different periods of up to 60 days. In addition, the prosthesis walls were evaluated with histological techniques in order to assess their integrity and the surrounding tissue reaction. Chitosan prostheses allowed PROG release during the time needed for nerve regeneration. At 45 days myelinated nerve fibers were observed in both the proximal and distal stumps. This parameter and the <jats:italic>N<\/jats:italic> ratio were higher in the progesterone\u2010treated group when compared to that of the vehicle control. Findings indicate that chitosan prostheses were useful in nerve regeneration, acting as a long\u2010lasting PROG delivery device a faster nerve regeneration. \u00a9 2003 Wiley Periodicals, Inc. 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