{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:26:33Z","timestamp":1775003193706,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,5,17]],"date-time":"2004-05-17T00:00:00Z","timestamp":1084752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2004,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study evaluated the nanoleakage patterns in bonded interfaces using two single\u2010step, self\u2010etching adhesives (Adper Prompt\u2014AD, and One\u2010up Bond F\u2014OB), two two\u2010step, self\u2010etching primers (Clearfil SE Bond\u2014CF, and Unifil Bond\u2014UB), and one two\u2010step, total\u2010etch adhesive (Single Bond\u2014SB). Dentin surfaces were bonded with the adhesive systems and stored in water at 37\u00b0C for 1 week and 6 months. After storage periods, teeth were sectioned into 0.8 mm\u2010thick slabs, coated with nail varnish except for the bonded interfaces, and immersed in ammoniacal AgNO<jats:sub>3<\/jats:sub> for 24 h. After immersion in photodeveloping solution, bonded sections were prepared and observed under a SEM using the backscattered electron mode. Undemineralized, unstained, epoxy resin\u2010embedded sections were prepared for TEM. Nanoleakage patterns were qualitatively compared between periods. Nanoleakage was observed in all bonded specimens at both periods. CF and UB presented silver deposits predominantly restricted to the thin (0.5 \u03bcm) hybrid layer (HL) at both periods. Although no evident differences were observed in the nanoleakage pattern of UB at 7 days and 6 months, CF presented enlarged areas of silver impregnation after 6 months. SB presented accumulation of silver particles mostly within the HL at 7 days, which was intensified after 6 months. AD and OB presented massive silver accumulation within the HL and the overlying adhesive layer. No evident differences were noticed between storage periods. Silver impregnation increased for all adhesive systems from 7 days to 6 months, except for UB. \u00a9 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 71B: 90\u201398, 2004<\/jats:p>","DOI":"10.1002\/jbm.b.30076","type":"journal-article","created":{"date-parts":[[2004,5,17]],"date-time":"2004-05-17T16:00:58Z","timestamp":1084809658000},"page":"90-98","source":"Crossref","is-referenced-by-count":45,"title":["Ultramorphological analysis of resin\u2013dentin interfaces produced with water\u2010based single\u2010step and two\u2010step adhesives: Nanoleakage expression"],"prefix":"10.1002","volume":"71B","author":[{"given":"Andr\u00e9 F.","family":"Reis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar A.G.","family":"Arrais","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro D.","family":"Novaes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo M.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rio F.","family":"De Goes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo","family":"Giannini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(71)90118-X"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820160307"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0109-5641(96)80033-9"},{"key":"e_1_2_6_5_2","first-page":"39","article-title":"Resin bonding to wet substrate. 1. 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