{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:56:41Z","timestamp":1773280601457,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T00:00:00Z","timestamp":1106784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to compare the nanoleakage patterns of the resin\u2013dentin interfaces of three dentin bonding systems at both TEM and field emission in lens SEM (FEI\u2010SEM) levels. A standardized smear layer was created with 180\u2010grit silicon carbide paper (SiC) on dentin disks obtained from 18 noncarious human third molars. Specimens were randomly divided into three groups and bonded with a two\u2010step total etching adhesive (Single Bond, SB), a two\u2010step, self\u2010etching adhesive (Clearfil SE BOND, SEB), and a one\u2010step, self\u2010etching adhesive (XENO III, XEIII). Nanoleakage was evaluated by using an ammoniacal silver\u2010nitrate solution. Specimens were processed for TEM and FEI\u2010SEM observation. The TEM of SB revealed silver deposits in adhesive and hybrid layers (HL). High\u2010magnification FEI\u2010SEM micrographs clearly identified these deposits as spherical clusters mainly associated with nonembedded collagen fibrils. TEM and FEI\u2010SEM examination of SEB revealed some clusters of silver deposits within porosities and small channels of the HL. Additional silver deposits were observed between the peritubular dentin walls and the resin tags. XEIII revealed very fine and diffuse silver grains throughout the entire HL. SEM visualization of nanoleakage at a high level of resolution has not been previously described. FEI\u2010SEM technology supported the TEM visualization with three\u2010dimensional morphological data of the relations between the HL constituents and nanoleakage. The results of the present study confirm the hypothesis that both total\u2010 and self\u2010etch adhesives are not able to fully infiltrate the dentin substrate. \u00a9 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 73B: 7\u201314, 2005<\/jats:p>","DOI":"10.1002\/jbm.b.30217","type":"journal-article","created":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T20:47:17Z","timestamp":1106858837000},"page":"7-14","source":"Crossref","is-referenced-by-count":55,"title":["Nanoleakage within the hybrid layer: A correlative FEISEM\/TEM investigation"],"prefix":"10.1002","volume":"73B","author":[{"given":"Pietro","family":"Suppa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Breschi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Ruggeri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Mazzotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Prati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Chersoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Di Lenarda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Pashley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin R.","family":"Tay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820160307"},{"key":"e_1_2_6_3_2","first-page":"3D","article-title":"Microscopy investigations. 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