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However, patients with lacrimal insufficiencies such as Stevens\u2010Johnson syndrome, chemical and thermal injuries, or ocular cicatricial pemphigoid have very limited options because of the short duration and action of lubricating agents. As a therapeutic strategy, we are working to develop a bioengineered tear secretory system for such patients. This article describes the growth and physiological properties of purified rabbit lacrimal gland acinar cells (pLGACs) on several matrix protein\u2010coated polymers such as silicone, collagen I, copolymers of poly\u2010<jats:sc>D,L<\/jats:sc>\u2010lactide\u2010<jats:italic>co<\/jats:italic>\u2010glycolide (PLGA; 85:15 and 50:50), poly\u2010<jats:sc>L<\/jats:sc>\u2010lactic acid (PLLA), and Thermanox\u00ae plastic cell culture coverslips. Monolayers of acinar cells were established on all of the polymeric substrata. An assay of \u03b2\u2010hexosaminidase activity in the supernatant medium showed significant increases in protein secretion, following stimulation with 100 \u03bc<jats:italic>M<\/jats:italic> carbachol on matrix protein\u2010coated and uncoated polymers such as silicone, PLGA 85:15, and PLLA. Our study demonstrates that PLLA supported the morphological and physiological properties of purified rabbit lacrimal gland epithelial cells more successfully than the others. \u00a9 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007<\/jats:p>","DOI":"10.1002\/jbm.b.30584","type":"journal-article","created":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T20:47:52Z","timestamp":1153255672000},"page":"192-200","source":"Crossref","is-referenced-by-count":22,"title":["Tissue\u2010engineered tear secretory system: Functional lacrimal gland acinar cells cultured on matrix protein\u2010coated substrata"],"prefix":"10.1002","volume":"80B","author":[{"given":"Shivaram","family":"Selvam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Padmaja B.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melvin 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