{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T05:35:37Z","timestamp":1736573737054,"version":"3.32.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,9,12]],"date-time":"2006-09-12T00:00:00Z","timestamp":1158019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reliable encapsulation of hemoglobin (Hb) within polypeptide multilayer nanofilms has been achieved by a template\u2010based approach, and protein functionality has been demonstrated postencapsulation. The method is general in scope and could be useful for many other encapsulants. Met\u2010Hb was adsorbed onto 5 \u03bcm\u2010diameter CaCO<jats:sub>3<\/jats:sub>microparticles, and the Hb\u2010coated particles were encapsulated within a multilayer nanofilm of poly(<jats:sc>L<\/jats:sc>\u2010glutamic acid) (PLGA) and poly(<jats:sc>L<\/jats:sc>\u2010lysine) (PLL) by layer\u2010by\u2010layer assembly. The CaCO<jats:sub>3<\/jats:sub>templates were then dissolved within the PLGA\/PLL nanofilms by addition of ethylenediaminetetraacetic acid. Encapsulation of Hb was proved by fluorescence microscopy, the pH\u2010dependence of retention of Hb was determined by visible wavelength absorbance, and conversion of the encapsulated met\u2010Hb to deoxy\u2010Hb and oxy\u2010Hb was demonstrated by spectroscopic analysis of the Soret absorption peak under various conditions. It thus has been shown that control of Hb oxygenation within polypeptide multilayer nanofilm artificial cells is possible, and that Hb thus encapsulated can bind, release, and subsequently rebind molecular oxygen. This work therefore represents an advance in the development of polypeptide multilayer film artificial red blood cells. \u00a9 2006 Wiley Periodicals, Inc. 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