{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T02:43:16Z","timestamp":1773542596818,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,4,26]],"date-time":"2007-04-26T00:00:00Z","timestamp":1177545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two bisphosphonates (BPs), namely 1\u2010hydroxy\u20102\u2010[4\u2010aminophenyl]ethane\u20101,1\u2010diphosphonic acid (APBP) and 1\u2010hydroxy\u20102\u2010[3\u2010indolyl]ethane\u20101,1\u2010diphosphonic acid (IBP), have been synthesized and incorporated to acrylic injectable and self\u2010curing formulations. Alendronic acid monosodium trihydrated salt (ALN) containing cement was formulated as control. These systems have potential applications in low density hard tissues affected by ailments characterized by a high osteoclastic resorption, i.e. osteoporosis and osteolysis. Values of curing parameters of APBP and IBP were acceptable to obtain pastes with enough fluency to be injected through a biopsy needle into the bone cavity. Working times ranged between 8 and 15 min and maximum temperature was around 50\u00b0C. Cured systems stored for a month in synthetic body fluid had compressive strengths between 90 and 96 MPa and modulus between 1.2 and 1.3 GPa, which suggest mechanical stabilization after setting and in the short time. BPs were released in PBS at an initial rate depending on the corresponding chemical structure in the order ALN &gt; APBP &gt; IBP to give final concentrations in PBS of 2.21, 0.44, and 0.19 mol\/mL for ALN, APBP, and IBP, respectively. Cytotoxicities of bisphosphonates were evaluated, IC<jats:sub>50<\/jats:sub> values being in the order APBP &gt; ALN &gt; IBP. Absence of cytotoxicity coming from leachables of the cured systems was observed in all cases independently of the BP. An improved cell growth and proliferation for the systems loaded with APBP and IBP compared with that loaded with ALN was observed, as assessed by measuring cell adhesion and proliferation, and total DNA content. \u00a9 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007<\/jats:p>","DOI":"10.1002\/jbm.b.30833","type":"journal-article","created":{"date-parts":[[2007,4,26]],"date-time":"2007-04-26T22:21:49Z","timestamp":1177626109000},"page":"596-608","source":"Crossref","is-referenced-by-count":8,"title":["Acrylic injectable and self\u2010curing formulations for the local release of bisphosphonates in bone tissue"],"prefix":"10.1002","volume":"83B","author":[{"given":"L. M.","family":"Rodr\u00edguez\u2010Lorenzo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Parra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"V\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"L\u00f3pez\u2010Bravo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. San","family":"Rom\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.38.1.375"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650101008"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20000615)88:12 <2961::AID-CNCR12>3.0.CO;2-L"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.11.024"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2005.pto_176.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5122(02)00145-7"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00061-2"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/10611869709017894"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.05.019"},{"key":"e_1_2_7_11_2","unstructured":"SimpsonH AthanasouN YatesAJ. Bisphosphonate cement composition to prevent aseptic loosening of orthopaedic implant devices. Patent WO 96\u2010US8515 19960603 1996."},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.57.10.614"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-200604000-00003"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.10296"},{"key":"e_1_2_7_15_2","unstructured":"BenedictJJ DegenhardtCR PoserJW. Diphosphonate\u2010derivatized macromolecules. US Patent 5011913;1991."},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(96)82725-6"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00130a036"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.10013"},{"key":"e_1_2_7_19_2","unstructured":"International Standard ISO 5833.Implants for Surgery\u2010Acrylic Resins Cements.1992."},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00701083"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00028-3"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90521-8"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10870-005-4551-z"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00314-3"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19971215)37:4<465::AID-JBM4>3.0.CO;2-J"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.03.010"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jab.770030105"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.10032"},{"key":"e_1_2_7_30_2","first-page":"613","article-title":"Cytotoxicity test of dental monomers using serum\u2010free cell culture (in vitro)","volume":"12","author":"Takeda S","year":"1993","journal-title":"J Jpn Dent Mater"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19971215)37:4<517::AID-JBM10>3.0.CO;2-5"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345880670111501"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.b.30833","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.30833","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T18:18:10Z","timestamp":1696875490000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.30833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,26]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1002\/jbm.b.30833"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.30833","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,26]]}}}