{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:21:33Z","timestamp":1791159693161,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T00:00:00Z","timestamp":1218067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new bioactive titanium surface treatment, labeled Ti\u2010ASD, was developed using the electrochemical anodic spark deposition (ASD) technique and results in a thickened titanium oxide layer with higher levels of calcium and phosphorus typical of newly deposited mineral phase. This study was aimed at extending the knowledge on Ti\u2010ASD treatment, by means of evaluation of the attachment, morphology, proliferation, metabolic activity, differentiation, and mineralization of osteoblast\u2010like cells (SaOS\u20102) after growth on Ti\u2010ASD treated titanium compared with nontreated titanium (Ti) and with chemically etched titanium (Ti\u2010ETC). This novel type of titanium coating supported cell attachment, cell proliferation, and mineralization, revealing no cytotoxicity effects. The expression of differentiation markers on Ti\u2010ASD treated titanium shows that genes related to the proliferation phase (Collagen type I, Coll I; Cbfa\u20101) were early expressed, whereas genes related to the mineralization phase (alkaline phosphatase, osteopontin, bone sialo protein) increased in a time\u2010related way. Mineralization occurred on all analyzed surfaces, but on Ti\u2010ASD the number of bone\u2010like nodules and the amount of mineralized area was higher. In conclusion, Ti\u2010ASD resulted to be a good surface for osteoblast attachment and proliferation, also promoting the maintenance of cell differentiation and matrix mineralization, a fundamental requirement for sustain the osseointegration and the clinical success of dental implants. \u00a9 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009<\/jats:p>","DOI":"10.1002\/jbm.b.31179","type":"journal-article","created":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T14:33:14Z","timestamp":1218119594000},"page":"280-289","source":"Crossref","is-referenced-by-count":14,"title":["Attachment, proliferation and osteogenic response of osteoblast\u2010like cells cultured on titanium treated by a novel multiphase anodic spark deposition process"],"prefix":"10.1002","volume":"88B","author":[{"given":"Elena","family":"De Angelis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Ravanetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Cacchioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attilio","family":"Corradi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Giordano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Candiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Chiesa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Gabbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Borghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,7]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"11","article-title":"The long\u2010term efficacy of currently used dental implants. 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