{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:35:34Z","timestamp":1784129734777,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,5,18]],"date-time":"2010-05-18T00:00:00Z","timestamp":1274140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Objectives: To characterize the interaction of 1\u2010Ethyl\u20103\u2010[3\u2010dimethylaminopropyl] carbodiimide Hydrochloride (EDC) with dentin matrix and its effect on the resin\u2010dentin bond. Methods: Changes to the stiffness of demineralized dentin fragments treated with EDC\/<jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide (NHS) in different solutions were evaluated at different time points. The resistance against enzymatic degradation was indirectly evaluated by ultimate tensile strength (UTS) test of demineralized dentin treated or not with EDC\/NHS and subjected to collagenase digestion. Short\u2010 and long\u2010term evaluations of the strength of resin\u2010dentin interfaces treated with EDC\/NHS for 1 h were performed using microtensile bond strength (\u03bcTBS) test. All data (MPa) were individually analyzed using ANOVA and Tukey HSD tests (<jats:italic>\u03b1<\/jats:italic> = 0.05). Results: The different exposure times significantly increased the stiffness of dentin (<jats:italic>p<\/jats:italic> &lt; 0.0001, control\u20105.15 and EDC\/NHS\u201029.50), while no differences were observed among the different solutions of EDC\/NHS (<jats:italic>p<\/jats:italic> = 0.063). Collagenase challenge did not affect the UTS values of EDC\/NHS group (6.08) (<jats:italic>p<\/jats:italic> &gt; 0.05), while complete degradation was observed for the control group (<jats:italic>p<\/jats:italic> = 0.0008, control\u201020.84 and EDC\/NHS\u201043.15). EDC\/NHS treatment did not significantly increase resin\u2010dentin \u03bcTBS, but the values remained stable after 12 months water storage (<jats:italic>p<\/jats:italic> &lt; 0.05). Conclusions: Biomimetic use of EDC\/NHS to induce exogenous collagen cross\u2010links resulted in increased mechanical properties and stability of dentin matrix and dentin\u2010resin interfaces. \u00a9 2010 Wiley Periodicals, Inc. 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