{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:38:27Z","timestamp":1759991907456},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,1,28]],"date-time":"2012-01-28T00:00:00Z","timestamp":1327708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nickel\u2010based alloys are used for dental restorations because of their strength, high moduli, and relatively low cost. However, these alloys corrode significantly in use, particularly in lower pH environments that are common under oral biofilms. Ni(II) corrosion products increase inflammatory cytokine secretion from activated monocytes, suggesting that nickel alloys may exacerbate inflammatory responses in adjacent periodontal tissues caused by dental plaque. Because inflammatory cytokine secretion is regulated in part by the NF\u03baB signaling pathway, our goal in the current work was to determine whether Ni(II) altered cellular levels or nuclear localization of NF\u03baB\u2010family subtypes. THP1 monocytes were exposed to Ni(II) for 72 h, and activated with lipopolysaccharide for the last 30 min to 6 h. Secretion of IL6 and TNF\u03b1 were measured using ELISA, and NF\u03baB levels and localization was measured using SDS\u2010PAGE with immunoblots and digital analysis. We observed that Ni(II) did not alter the levels of secreted TNF\u03b1 from activated monocytes, but increased secreted IL6 levels about 30% over controls. Ni(II) did not alter whole\u2010cell levels of any NF\u03baB subtype, but increased nuclear persistance of p65 and c\u2010Rel. Our results suggest that Ni(II) may increase inflammatory cytokine secretion by increasing nuclear localization of some NF\u03baB subtypes. Further studies should be done to determine the prominence of this mechanism in clinical environments. \u00a9 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.<\/jats:p>","DOI":"10.1002\/jbm.b.32655","type":"journal-article","created":{"date-parts":[[2012,1,28]],"date-time":"2012-01-28T06:44:46Z","timestamp":1327733086000},"page":"934-939","source":"Crossref","is-referenced-by-count":8,"title":["Ni(II) alters the NF\u03baB signaling pathway in monocytic cells"],"prefix":"10.1002","volume":"100B","author":[{"given":"Lei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Wataha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Casey","family":"Cate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"DiJulio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Whasun O.","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2003.12.010"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3913(85)80087-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016359109005889"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jab.770050305"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/397029"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3913(98)70057-5"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1002\/jbm.1052","article-title":"Relating nickel\u2010induced tissue inflammation to nickel release in vivo","volume":"58","author":"Wataha JC","year":"2001","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.20029"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.31063"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/515178"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00002-3"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2008.12.003"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.5.2327"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(02)00777-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.30383"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10169"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.06.007"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10352"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2083"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb01649.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.7.4443"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.12.008"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100864"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09283"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.395"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.b.32655","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.32655","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T07:25:29Z","timestamp":1697527529000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.32655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,28]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/jbm.b.32655"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.32655","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,28]]}}}