{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:45:01Z","timestamp":1791377101489,"version":"4.3.2"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T00:00:00Z","timestamp":1382313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In addressing the issue of prosthetic infection, this work demonstrated the synergistic effect of the application of static magnetic field (SMF) and ferrimagnetic substrate properties on the bactericidal property <jats:italic>in vitro<\/jats:italic>. This aspect was studied using hydroxyapatite (HA)\u2010xFe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> (x=10, 20, and 40 wt.%) substrates, which have different saturation magnetization properties. During bacteria culture experiments, 100 mT SMF was applied to growth medium (with HA\u2010xFe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> substrate) <jats:italic>in vitro<\/jats:italic> for 30, 120, and 240 min. A combination of MTT assay, membrane rupture assays, live\/dead assay, and fluorescence microscopic analysis showed that the bactericidal effect of SMF increases with the exposure duration as well as increasing Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> content in biomaterial substrates. Importantly, the synergistic bactericidal effect was found to be independent of bacterial cell type, as similar qualitative trend is measured with both gram negative <jats:italic>Escherichia coli (E. coli)<\/jats:italic> and gram positive <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S. aureus<\/jats:italic>) strains. The reduction in <jats:italic>E. coli<\/jats:italic> viability was 83% higher on HA\u201040 Wt % Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> composite after 4 h exposure to SMF as compared to nonexposed control. Interestingly, any statistically significant difference in ROS was not observed in bacterial growth medium after magnetic field exposure, indicating the absence of ROS enhancement due to magnetic field. Overall, this study illustrates significant role being played by magnetic substrate compositions towards bactericidal property than by magnetic field exposure alone. \u00a9 2013 Wiley Periodicals, Inc. 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