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Most of these steps require extensive use of chemicals\/reagents\/enzymes which may also adversely affect the quality of the scaffold. With an effort to minimize the use of chemicals\/reagents\/enzymes, while extracting biomaterial\u2010grade ECM from porcine cholecyst (gall bladder), we performed preisolation <jats:italic>ex situ<\/jats:italic> incubation of the organ in a stabilizing agent that also caused <jats:italic>in situ<\/jats:italic> crosslinking of tissue\u2010components and delaminated the collagen\u2010rich ECM from the tissue\u2010layer beneath the mucosa. The physical, chemical, and biological properties of the isolated scaffolds were similar to that of a commercially available porcine small intestinal submucosa. The cholecyst\u2010derived scaffold not only satisfied preclinical safety\u2010test procedures such as cytotoxicity, local response, and endotoxin load but also showed the potential to promote healing of full\u2010thickness skin wound in a rabbit model. The procedure was also suitable for isolating scaffolds from other hollow organs such as jejunum and urinary bladder. It was concluded that enzyme\/detergent treatment may be an avoidable step while isolating biomaterial\u2010grade scaffolds from hollow organs. \u00a9 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1506\u20131516, 2014.<\/jats:p>","DOI":"10.1002\/jbm.b.33131","type":"journal-article","created":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T07:26:42Z","timestamp":1394004402000},"page":"1506-1516","source":"Crossref","is-referenced-by-count":30,"title":["Biomaterial properties of cholecyst\u2010derived scaffold recovered by a non\u2010detergent\/enzymatic method"],"prefix":"10.1002","volume":"102","author":[{"given":"Thapasimuthu V.","family":"Anilkumar","sequence":"first","affiliation":[{"name":"Division of Experimental Pathology Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology Trivandrum 695012 Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadavanath P.","family":"Vineetha","sequence":"additional","affiliation":[{"name":"Division of Experimental Pathology Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology Trivandrum 695012 Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepa","family":"Revi","sequence":"additional","affiliation":[{"name":"Division of Experimental Pathology Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology Trivandrum 695012 Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaseer","family":"Muhamed","sequence":"additional","affiliation":[{"name":"Division of Experimental Pathology Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology Trivandrum 695012 Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akhila","family":"Rajan","sequence":"additional","affiliation":[{"name":"Division of Experimental Pathology Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology Trivandrum 695012 Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.09.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.04.043"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3182445341"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm061088x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/8\/1\/014106"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34764"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34708"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34083"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064134"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064151"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.09.017"},{"key":"e_1_2_7_13_1","first-page":"3675","article-title":"Decellularization of tissues and organs","volume":"27","author":"Gilbert TW","year":"2006","journal-title":"Biomaterials"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30170"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.057"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/620503"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002890050404"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2008.02.013"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/1045-1056(95)90014-4"},{"key":"e_1_2_7_20_1","volume-title":"ISO 10993\u20105:2009. 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