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However, the pericardial tissue employed to this aim undergoes severe calcification due to chronic inflammation resulting from a non\u2010complete immunological compatibility of the animal\u2010derived pericardial tissue resulting from failure to remove animal\u2010derived xeno\u2010antigens. In the mid\/long\u2010term, this leads to structural deterioration, mechanical failure, and prosthesis leaflets rupture, with consequent need for re\u2010intervention. In the search for novel procedures to maximize biological compatibility of the pericardial tissue into immunocompetent background, we have recently devised a procedure to decellularize the human pericardium as an alternative to fixation with aldehydes. In the present contribution, we used this procedure to derive sheets of decellularized pig pericardium. The decellularized tissue was first tested for the presence of 1,3 \u03b1\u2010galactose (\u03b1Gal), one of the main xenoantigens involved in prosthetic valve rejection, as well as for mechanical tensile behavior and distensibility, and finally seeded with pig\u2010 and human\u2010derived aortic valve interstitial cells. We demonstrate that the decellularization procedure removed the \u03b1GAL antigen, maintained the mechanical characteristics of the native pig pericardium, and ensured an efficient surface colonization of the tissue by animal\u2010 and human\u2010derived aortic valve interstitial cells. This establishes, for the first time, the feasibility of fixative\u2010free pericardial tissue seeding with valve competent cells for derivation of tissue engineered heart valve leaflets. \u00a9 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 345\u2013356, 2016.<\/jats:p>","DOI":"10.1002\/jbm.b.33404","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T17:02:49Z","timestamp":1427216569000},"page":"345-356","source":"Crossref","is-referenced-by-count":17,"title":["Feasibility of pig and human\u2010derived aortic valve interstitial cells seeding on fixative\u2010free decellularized animal pericardium"],"prefix":"10.1002","volume":"104","author":[{"given":"Rosaria","family":"Santoro","sequence":"first","affiliation":[{"name":"Unit\u00e0 di Ingegneria Tissutale Centro Cardiologico Monzino, IRCCS Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Consolo","sequence":"additional","affiliation":[{"name":"Dipartimento di Elettronica Informazione e Bioingegneria, Politecnico di Milano Milano 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