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This motivates tissue\u2010engineering researchers to develop scaffolds as artificial matrices that give mechanical support for osteoblasts, cementoblasts, gingival and periodontal ligament fibroblast cells. In this study, modified hyaluronic acid (HA) and chitosan (CS) were employed to create a hybrid CS\u2010HA hydrogel scaffold for periodontal regeneration. CS, HA, and CS\u2010HA scaffolds were obtained by freeze\u2010drying technique, resulting in porous structures suitable for use in tissue engineering. Scaffolds were submitted to gamma and UV\u2010sterilization without significant morphology changes. The ATR\u2010FTIR spectra of CS\u2010HA hydrogels showed peaks at 377 cm<jats:sup>\u22121<\/jats:sup>, 1566 cm<jats:sup>\u22121<\/jats:sup>, and 1614 cm<jats:sup>\u22121<\/jats:sup>, representing secondary amide, primary amine, and carboxyl acid respectively, and it was also observed the emergence of peaks at 886 cm<jats:sup>\u22121<\/jats:sup>, which probably represents the Schiff base formed in the case of hybrid CS\u2010HA hydrogels. The scaffolds presented a high rate of PBS uptake, reaching values higher than 95%. Thermal degradation of HA scaffolds was around 225\u00b0C and CS was around 285\u00b0C. The ATR\u2010FTIR spectra and swelling degree were slightly disturbed mainly after gamma sterilization, but degradation temperature did not change after sterilization. The performance of the CS\u2010HA hydrogel scaffolds for <jats:italic>in vitro<\/jats:italic> cell culture was tested using NIH3T3 and MG63 cell lines. The Alamar Blue test showed a significant increase in cellular viability and high CD44 expression, suggesting that the cells migrated more when seeded onto the scaffolds. \u00a9 2015 Wiley Periodicals, Inc. 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